PMID: 8610126Apr 2, 1996Paper

G protein subunits and the stimulation of phospholipase C by Gs-and Gi-coupled receptors: Lack of receptor selectivity of Galpha(16) and evidence for a synergic interaction between Gbeta gamma and the alpha subunit of a receptor activated G protein

Proceedings of the National Academy of Sciences of the United States of America
X Zhu, L Birnbaumer

Abstract

Stimulatory guanine nucleotide binding protein (Gs)-coupled receptors activated by luteinizing hormone, vasopressin, and the catecholamine isoproterenol (luteinizing hormone receptor, type 2 vasopressin receptor, and types 1 and 2 beta-adrenergic receptors) and the Gi-coupled M2 muscarinic receptor (M2R) were expressed transiently in COS cells, alone and in combination with Gbeta gamma dimers, their corresponding Galphas (Galpha(s), or Galpha(i3)) and either Galpha(q) or Galpha(16). Phospholipase C (PLC) activity, assessed by inositol phosphate production from preincorporated myo[3H]inositol, was then determined to gain insight into differential coupling preferences among receptors and G proteins. The following were observed: (i) All receptors tested were able to stimulate PLC activity in response to agonist occupation. The effect of the M2R was pertussis toxin sensitive. (ii) While, as expected, expression of Galpha(q) facilitated an agonist-induced activation of PLC that varied widely from receptor to receptor (400% with type 2 vasopressin receptor and only 30% with M2R), expression of Galpha(16) facilitated about equally well the activation of PLC by any of the tested receptors and thus showed little if any discrimination fo...Continue Reading

References

Sep 18, 1992·Cell·W J Tang, A G Gilman
May 7, 1990·Biochimica Et Biophysica Acta·L BirnbaumerA M Brown
Jun 23, 1995·The Journal of Biological Chemistry·S Offermanns, M I Simon
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D WuM I Simon

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Citations

Aug 21, 2013·Gene·Rute S T MartinsAdelino V M Canário
Jan 15, 1999·Pharmacology & Therapeutics·J Wess
Nov 8, 2001·Trends in Pharmacological Sciences·E Kostenis
May 19, 1998·Trends in Pharmacological Sciences·L A Selbie, S J Hill
Dec 6, 2002·British Journal of Pharmacology·Nicole SawyerFrancois G Gervais
Aug 27, 2011·Chemical Senses·Maria Mercedes GalindoMaik Behrens
Sep 5, 2002·Annual Review of Physiology·Jürgen Schnermann, David Z Levine
Nov 25, 2011·American Journal of Physiology. Renal Physiology·Edwin K JacksonZaichuan Mi
Sep 27, 2008·PLoS Computational Biology·Patrick FlahertyAdam P Arkin
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·B VannierL Birnbaumer
Sep 1, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·O A ThometH U Simon
Dec 25, 2009·Fundamental & Clinical Pharmacology·Britta HaenischHeinz Bönisch
Aug 1, 1997·Biochemical Pharmacology·J D Hildebrandt
Jul 15, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Patricia ReuquénPedro A Orihuela
Oct 20, 2005·Journal of Biomolecular Screening·Wayne R LeifertEdward J McMurchie
Mar 21, 2003·Journal of Cellular Biochemistry·Jennifer L GuMingyao Liu
Jan 15, 2005·Journal of Receptor and Signal Transduction Research·Peter J K Knight, Thomas A Grigliatti
Sep 26, 2007·Journal of Neuroscience Research·Saeed Esmaeili-MahaniAbolhasan Ahmadiani
Oct 27, 2006·The Journal of Pharmacology and Experimental Therapeutics·P MichalV Dolezal
Oct 26, 2000·The Journal of Biological Chemistry·P CasarosaM J Smit
Jun 13, 1997·The Journal of Biological Chemistry·S G Rhee, Y S Bae
Sep 12, 2019·Scientific Reports·Aykut Göktürk ÜnerYoung-Bum Kim
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·U Quitterer, M J Lohse

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