PMID: 9438382Jan 23, 1998Paper

G proteins and regulation of pyruvate dehydrogenase activity by insulin in human circulating lymphocytes

The International Journal of Biochemistry & Cell Biology
M CurtoMaria T Rinaudo

Abstract

Pertussis toxin (PT) catalyzes ADP-ribosylation of G protein alpha subunits, thus preventing their role as transducers of external signals targeting metabolic pathways. In vitro, in human circulating lymphocytes insulin at physiological concentrations (5 microU/ml) determines sharp activation of pyruvate dehydrogenase (PDH), the rate limiting enzyme in glucose oxidative breakdown. This study evaluates whether the above-described effects of insulin over PDH are mediated through G proteins. Human circulating lymphocytes (six samples from different donors) were exposed to insulin (5 microU/ml), PT (1-2 micrograms/ml) or PT-9K, a mutated PT void of catalytic activity (1-10 micrograms/ml), and to insulin in combination with the two toxins, and then assessed for PDH activity. Plasma membranes from cells incubated with and without PT or PT-9K were subjected to ADP-ribosylation in the presence of [32P] NAD+ and activated PT. In circulating lymphocytes exposed to PT alone, or in combination with insulin, PDH activity falls significantly below basal values (P < 0.001); PT-9K instead has no effect on basal or on insulin-stimulated PDH activity. ADP-ribosylation of a plasma membrane component with apparent molecular mass (42 kDa) comparabl...Continue Reading

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Citations

Apr 10, 2007·Archivum Immunologiae Et Therapiae Experimentalis·Paweł PiatkiewiczJan Tatoń
Nov 4, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Anna CzechJan Tatoń
Jul 6, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·M PiccininiM T Rinaudo

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