PMID: 2506760Sep 1, 1989Paper

G proteins mediate suppression of Ca2+-activated K current by acetylcholine in smooth muscle cells

The American Journal of Physiology
W C Cole, K M Sanders

Abstract

The role of G proteins in cholinergic suppression of Ca2+-activated K current was studied in isolated canine colonic myocytes with the whole cell voltage-clamp technique. Acetylcholine (ACh; 10.0 microM) caused a 64 +/- 2.4% depression in the Ca2+-dependent component of the outward current evoked at potentials between -45 and -15 mV when GTP (0.1 microM) was included in the pipette-filling solution. This effect was reversed within 2-4 min on washout of ACh. Without GTP in the filling solution, ACh caused a 15 +/- 2.5% depression in outward current in 60% of the cells tested. When the non-hydrolyzable GTP analogues, GTP gamma S (0.1 mM) or 5'-guanylylimidodiphosphate (GppNHp; 0.1 mM) were used, the decrease in outward current was greater (85 +/- 4.2 and 78 +/- 6.5%, respectively), and it was not reversed on withdrawal of ACh. Dialysis of the cell interior with pipette solution containing pertussis toxin (1 ng/ml) for 30 min had no effect on the whole cell currents evoked on depolarization, but it abolished the effect of ACh on Ca2+-dependent outward current. These data suggest that coupling of muscarinic receptors to the inhibition of Ca2+-activated K channels is mediated by pertussis toxin-sensitive G proteins in colonic smooth...Continue Reading

References

Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L H ClappJ J Singer
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
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Citations

Aug 1, 1991·Journal of Bioenergetics and Biomembranes·L Toro, E Stefani
Dec 21, 2002·International Journal of Urology : Official Journal of the Japanese Urological Association·Takayuki NakamuraOsamu Yamaguchi
May 25, 2013·American Journal of Physiology. Cell Physiology·Shankar P Parajuli, Georgi V Petkov
Aug 1, 1995·Pflügers Archiv : European journal of physiology·T NakajimaY Kurachi
Aug 1, 1991·Journal of Bioenergetics and Biomembranes·O B McManus
Oct 1, 2013·Pflügers Archiv : European journal of physiology·Ligia ToroEnrico Stefani
Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
Feb 2, 1999·American Journal of Respiratory and Critical Care Medicine·H Kume, K Takagi
Aug 1, 1995·British Journal of Pharmacology·N A Parkinson, A D Hughes
Nov 1, 1992·Pharmacology & Therapeutics·L MissiaenR Casteels
Jan 1, 1993·Pharmacology & Therapeutics·M I Kotlikoff
Jan 4, 1994·European Journal of Pharmacology·S P OlesenJ Drejer

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