G-quadruplexes as novel cis-elements controlling transcription during embryonic development

Nucleic Acids Research
Aldana P DavidNora B Calcaterra

Abstract

G-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during vertebrate embryonic development has not yet been addressed. Here, we performed an in silico search to find conserved putative G-quadruplex sequences (PQSs) within proximal promoter regions of human, mouse and zebrafish developmental genes. Among the PQSs able to fold in vitro as G-quadruplex, those present in nog3, col2a1 and fzd5 promoters were selected for further studies. In cellulo studies revealed that the selected G-quadruplexes affected the transcription of luciferase controlled by the SV40 nonrelated promoter. G-quadruplex disruption in vivo by microinjection in zebrafish embryos of either small ligands or DNA oligonucleotides complementary to the selected PQSs resulted in lower transcription of the targeted genes. Moreover, zebrafish embryos and larvae phenotypes caused by the presence of complementary oligonucleotides fully resembled those ones reported for nog3, col2a1 and fzd5 morphants. To our knowled...Continue Reading

References

Oct 28, 1994·Science·L Wolpert
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam Siddiqui-JainLaurence H Hurley
Jul 15, 2004·Journal of the American Chemical Society·Jeyaprakashnarayanan SeenisamyLaurence H Hurley
May 26, 2005·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Jul 18, 2006·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Dec 16, 2006·Nucleic Acids Research·Julian L Huppert, Shankar Balasubramanian
Jun 5, 2007·Developmental Biology·John M GansnerJonathan D Gitlin
Mar 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Indika RajapakseSteven T Kosak
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tom Misteli
Jun 12, 2009·Current Opinion in Chemical Biology·Shankar Balasubramanian, Stephen Neidle
Sep 18, 2009·Nucleic Acids Research·Zhuo DuNing Li
Oct 15, 2009·The Journal of Physical Chemistry. B·Luigi MartinoConcetta Giancola
Mar 26, 2010·Disease Models & Mechanisms·Steve MangosIain A Drummond
Aug 21, 2010·Journal of Nucleic Acids·David MonchaudMarie-Paule Teulade-Fichou
Feb 22, 2011·Journal of Paediatrics and Child Health·Peter KannuRavi Savarirayan
Jul 26, 2011·Database : the Journal of Biological Databases and Curation·Rhoda J KinsellaPaul Flicek
May 3, 2012·Genome Research·Fabian A BuskeTimothy L Bailey
Jul 4, 2012·Topics in Current Chemistry·Antonio RandazzoMateus Webba da Silva
Oct 4, 2012·Nature Reviews. Genetics·Matthew L BochmanVirginia A Zakian
Jan 26, 2013·PloS One·Masataka NikaidoRobert N Kelsh
Feb 21, 2013·Nature Chemistry·Giulia BiffiShankar Balasubramanian
Jan 21, 2014·Biochimica Et Biophysica Acta·Pablo DomiziClaudia Banchio
Feb 11, 2014·Nucleic Acids Research·Amandine Renaud de la FaverieJean-Louis Mergny
Mar 4, 2014·Current Opinion in Genetics & Development·Pierre Murat, Shankar Balasubramanian
Aug 26, 2014·Biochimica Et Biophysica Acta·Ezequiel MargaritNora B Calcaterra
Jul 8, 2015·EMBO Reports·Nancy Maizels
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Aug 27, 2015·Nature·Ke ZhangJeffrey D Rothstein
Oct 10, 2015·BMC Medical Genetics·John A Smestad, L James Maher

❮ Previous
Next ❯

Citations

Oct 17, 2016·Neuropeptides·Olympia GianfrancescoJohn P Quinn
Jan 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-Yin SunTian-Miao Ou
Nov 23, 2019·Nucleic Acids Research·Emilia Puig Lombardi, Arturo Londoño-Vallejo
Apr 17, 2020·G3 : Genes - Genomes - Genetics·H Busra Cagirici, Taner Z Sen
Apr 13, 2018·Nucleic Acids Research·Marko TrajkovskiNaoki Sugimoto
Oct 10, 2018·Nature Nanotechnology·Diwei HoK Swaminathan Iyer
Mar 25, 2019·Scientific Reports·Bei LiDugarjaviin Manglai
Dec 30, 2017·Current Medicinal Chemistry·Nayun Kim
Jun 21, 2019·Nucleic Acids Research·Aldana P DavidPablo Armas
Mar 18, 2020·Nucleic Acids Research·Lukasz CiszewskiLinda Popplewell
Nov 4, 2017·Current Genetics·Eike Schwindt, Katrin Paeschke
Nov 14, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Paula Daniela MestizoJohn J Hurtado
Dec 18, 2020·Frontiers in Cell and Developmental Biology·Alexandra A KuznetsovaNikita A Kuznetsov
Mar 31, 2021·Nucleic Acids Research·Ernest WangJiou Wang
Apr 4, 2021·International Journal of Molecular Sciences·Georgina BezziPablo Armas
Apr 2, 2021·Biophysical Journal·Alexa M Salsbury, Justin A Lemkul
Nov 25, 2020·Seminars in Cell & Developmental Biology·Taylor A Evans, Jennifer Ann Erwin
Oct 24, 2018·ACS Synthetic Biology·Shinzi Ogasawara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.