Gα subunit coordinates with ephrin-B to balance self-renewal and differentiation in neural progenitor cells.

Stem Cells
Kiyohito MuraiQiang Lu

Abstract

Proper development of the mammalian brain requires that neural progenitor cells balance self-renewal and differentiation under precise temporal and spatial regulation, but the underlying mechanisms are not well understood. In this study, we identify Gα subunit as a positive regulator of mammalian neurogenesis, working with the regulator of G protein signaling (RGS)-mediated ephrin-B signaling pathway as two opposing forces to maintain a balance between self-renewal and differentiation in the developing mouse cerebral cortex. Multiple Gα(i) subunits are expressed by cortical neural progenitor cells during the course of cortical neurogenesis. Activation of Gα(i) signaling, through in utero electroporation-mediated expression of wild-type and constitutively active Gα(i) subunits, counteracts the function of ephrin-B in cortical neural progenitors to induce differentiation. Genetic knock-in of an RGS-insensitive G184SGα(i2) causes early cell cycle exit and a reduction of cortical neural progenitor cells and leads to a defect in the production of late born cortical neurons, similar to what is observed in mutant mice with deficiency in ephrin-B reverse signaling pathway. This study reveals a role of Gα subunit in mammalian neurogenes...Continue Reading

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Citations

Mar 9, 2013·Archives of Biochemistry and Biophysics·Srinivas RamasamySohail Ahmed
Dec 9, 2014·Cell Adhesion & Migration·J LaussuA Davy
Dec 9, 2014·Cell Adhesion & Migration·David G Wilkinson
Mar 12, 2015·Journal of Molecular Neuroscience : MN·Chao-Jin XuWei-Lin Jin
Mar 3, 2015·Frontiers in Cellular Neuroscience·Sara Bizzotto, Fiona Francis
May 15, 2020·BMC Developmental Biology·Anthony KischelAlice Davy
Aug 31, 2020·Cerebral Cortex Communications·Runxiang QiuQiang Lu
Apr 13, 2013·Development·Dina N ArvanitisAlice Davy

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