G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair

Molecular & General Genetics : MGG
D Lydall, T Weinert

Abstract

We have cloned, sequenced and disrupted the checkpoint genes RAD17, RAD24 and MEC3 of Saccharomyces cerevisiae. Mec3p shows no strong similarity to other proteins currently in the database. Rad17p is similar to Rec1 from Ustilago maydis, a 3' to 5' DNA exonuclease/checkpoint protein, and the checkpoint protein Rad1p from Schizosaccharomyces pombe (as we previously reported). Rad24p shows sequence similarity to replication factor C (RFC) subunits, and the S. pombe Rad17p checkpoint protein, suggesting it has a role in DNA replication and/or repair. This hypothesis is supported by our genetic experiments which show that overexpression of RAD24 strongly reduces the growth rate of yeast strains that are defective in the DNA replication/repair proteins Rfc1p (cdc44), DNA pol alpha (cdc17) and DNA pol delta (cdc2) but has much weaker effects on cdc6, cdc9, cdc15 and CDC4 strains. The idea that RAD24 overexpression induces DNA damage, perhaps by interfering with replication/repair complexes, is further supported by our observation that RAD24 overexpression increases mitotic chromosome recombination in CDC4 strains. Although RAD17, RAD24 and MEC3 are not required for cell cycle arrest when S phase is inhibited by hydroxyurea (HU), they...Continue Reading

Citations

Apr 15, 2003·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Hidefumi SasakiYoshitaka Fujii
Aug 1, 2000·Mutation Research·J E Haber
Aug 1, 2000·Mutation Research·T Humphrey
Sep 15, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·H SasakiY Fujii
Feb 11, 2004·FEMS Microbiology Letters·Sasha H ShafikhaniTerrance Leighton
Jul 19, 2001·Mutation Research·S BroomfieldW Xiao
Jan 23, 1999·Current Opinion in Cell Biology·N Rhind, P Russell
Jun 4, 1998·Current Opinion in Genetics & Development·T Weinert
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Salma KaocharTed Weinert
Mar 13, 2003·Molecular Biology of the Cell·Arne S IJpma, Carol W Greider
Nov 17, 1999·Molecular Biology of the Cell·M E Grether, I Herskowitz
Oct 12, 2000·Molecular Biology of the Cell·P A San-Segundo, G S Roeder
Oct 2, 1998·The EMBO Journal·M P LongheseP Plevani
Jul 6, 2010·Nucleic Acids Research·Christopher W Pitt, Julia Promisel Cooper
Dec 28, 2002·Genes & Development·Kenji ShimadaSusan M Gasser
Feb 5, 2002·Genes & Development·Eun-Jin Erica Hong, G Shirleen Roeder
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·T WeinertP Sundareshan
Apr 21, 2001·Molecular and Cellular Biology·M KaiT S Wang
Apr 5, 2001·Molecular and Cellular Biology·J S HannaF A Spencer
Jul 31, 2007·Genetics·Marenda A WilsonAmbro van Hoof
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vladimir P BermudezAziz Sancar
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·L A Lindsey-BoltzA Sancar
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jerzy Majka, Peter M J Burgers
Nov 5, 2010·Annual Review of Genetics·Devanshi Jain, Julia Promisel Cooper
Aug 15, 2014·Nucleic Acids Research·Greg H P NgoDavid Lydall
Aug 28, 2015·Molecular Biology of the Cell·Neil R AdamesJean Peccoud
Feb 14, 2009·DNA Repair·Linda B Bloom
Jul 2, 2005·Biochimie·Valeria ViscardiMaria Pia Longhese

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.