G2R Cre reporter transgenic zebrafish.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Shunichi YoshikawaXinping C Zhao

Abstract

The Cre/loxP site-specific recombination system has been widely used to manipulate DNA in vivo and to study gene function in the mouse by inducible transgenic and conditional gene targeting. To fully use this powerful genetic tool in a relatively new animal model, zebrafish, we generated reporter transgenic lines for easy detection of Cre recombinase activity in vivo. The transgenic fish lines, designated G2R, express two fluorescent protein genes, GFP and RFP, under the control of the ubiquitous promoter of the Xenopus EF1 alpha gene. The G2R animals change their color from green to red (G2R) after Cre-mediated recombination and are useful for development of cell type specific Cre transgenic lines and for cell lineage and fate mapping studies in zebrafish.

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Citations

Mar 29, 2013·Marine Biotechnology·Feng XiongYong-Hua Sun
Mar 17, 2010·Pigment Cell & Melanoma Research·E Elizabeth PattonRodney S Nairn
Mar 3, 2012·Yi chuan = Hereditas·Ding-Pei LongZhong-Huai Xiang
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Nov 26, 2013·Zebrafish·Thessicar Evadney AntoineVaibhav Tiwari
May 20, 2011·Developmental Neurobiology·Caghan KizilMichael Brand
Dec 1, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stefan HansMichael Brand
Jan 23, 2010·Genesis : the Journal of Genetics and Development·James A Lister
Mar 5, 2013·General and Comparative Endocrinology·Yuya Sugano, Stephan C F Neuhauss
Oct 26, 2018·Transgenic Research·Kusumika Mukherjee, Eric C Liao
May 28, 2011·Reviews in the Neurosciences·Claire Wyart, Filippo Del Bene

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