G9a stimulates CRC growth by inducing p53 Lys373 dimethylation-dependent activation of Plk1

Theranostics
Jie ZhangYi Chen

Abstract

Rationale: G9a is genetically deregulated in various tumor types and is important for cell proliferation; however, the mechanism underlying G9a-induced carcinogenesis, especially in colorectal cancer (CRC), is unclear. Here, we investigated if G9a exerts oncogenic effects in CRC by increasing polo-like kinase 1 (Plk1) expression. Thus, we further characterized the detailed molecular mechanisms. Methods: The role of Plk1 in G9a aberrant CRC was determined by performing different in vitro and in vivo assays, including assessment of cell growth by performing cell viability assay and assessment of signaling transduction profiles by performing immunoblotting, in the cases of pharmacological inhibition or short RNA interference-mediated suppression of G9a. Detailed molecular mechanisms underlying the effect of G9a on Plk1 expression were determined by performing point mutation analysis, chromatin immunoprecipitation analysis, and luciferase reporter assay. Correlation between G9a and Plk1 expression was determined by analyzing clinical samples of patients with CRC by performing immunohistochemistry. Results: Our study is the first to report a significant positive correlation between G9a and Plk1 levels in 89 clinical samples of patie...Continue Reading

Citations

Jan 22, 2020·Journal of Receptor and Signal Transduction Research·Yonglan ZhangWei Wang
Apr 7, 2020·Journal of Receptor and Signal Transduction Research·Wenjun LiangMingxia Yang
Dec 30, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Nirmalya Saha, Andrew G Muntean

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Methods Mentioned

BETA
transfection
immunoprecipitation
ChIP
PCR
Assay
xenograft
xenografts
co-immunoprecipitation
co-IP
acetylation

Software Mentioned

CalcuSyn
GraphPad Prism

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