GABA-activated single channel currents in outside-out membrane patches from rat retinal ganglion cells

Visual Neuroscience
S A Lipton

Abstract

gamma-aminobutyric acid (GABA) evokes large whole-cell currents in solitary mammalian retinal ganglion cells studied by the patch-clamp method. This evidence suggests that GABA acts directly on the retinal ganglion cells as an inhibitory transmitter as it does elsewhere in the mammalian central nervous system. Here, single-channel recordings of the currents underlying the GABA-induced responses were studied in outside-out patches of cell membrane. In some other preparations, single GABAA channels recorded in the excised patch configuration have been shown to have altered properties in comparison to responses elicited during whole-cell recording. For example, in cortical neurons single GABA-activated channels in excised patches display accelerated desensitization kinetics as well as rapid rundown of the response. Therefore, in retinal ganglion cells, responses generated by GABA in cell-free patches were compared to whole-cell responses. After determining that the responses to GABA in acutely isolated outside-out patches were indeed similar to those of the whole-cell currents in retinal ganglion cells, the unitary conductances were studied. It was determined that these single-channel events resemble those reported in other nervou...Continue Reading

References

Apr 1, 1987·The Journal of Physiology·S A Lipton, D L Tauck
Nov 1, 1989·The Journal of Physiology·A Karschin, S A Lipton
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J Bormann, D E Clapham
Sep 1, 1988·Nature·E S LevitanM G Darlison
Jan 1, 1987·Progress in Neurobiology·S C Massey, D A Redburn
Aug 1, 1988·Journal of Neurophysiology·A T Ishida, B N Cohen
Sep 1, 1987·Pflügers Archiv : European journal of physiology·S A LiptonR H Loring
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth

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Citations

Jul 1, 1993·Vision Research·X L Yang, S M Wu
May 3, 2005·Journal of Pharmacological and Toxicological Methods·Sarah Lilley, Jon Robbins
Apr 16, 1998·Progress in Retinal and Eye Research·A Feigenspan, J Bormann
Jan 13, 1998·Vision Research·N J SucherE B Dreyer
Feb 1, 1992·Visual Neuroscience·N J Sucher, S A Lipton
Sep 1, 1992·Visual Neuroscience·F MüllerH Wässle
May 1, 1993·Visual Neuroscience·U GreferathP Seeburg

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