PMID: 9182847Jun 30, 1997Paper

GABA immunoreactivity in hypothalamic neurons and growth cones in early development in vitro before synapse formation

The Journal of Comparative Neurology
A N van den Pol

Abstract

GABA (gamma-aminobutyrate) is the most prevalent inhibitory transmitter in the mature hypothalamus. In contrast, in the developing hypothalamus, GABA may exert depolarizing actions leading to neuronal excitation. To determine whether GABA is present in hypothalamic neurons early in development, and whether there is a preferential expression in axonal growth cones, immunogold and peroxidase studies were used with light and whole mount transmission electron microscopy. At embryonic day 15, a stage of development at the beginning of hypothalamic neurogenesis, histological sections showed GABA immunoreactivity in fibers and weakly stained perikarya. Hypothalamic neurons (13%) cultured at embryonic day 15 were immunoreactive after 1 day in vitro. The percentage of neurons stained, and the intensity of staining increased during the next few days to 39% at 4 days in vitro. Neuritic growth cones, including lamellipodia and long filopodia, showed strong immunoreactivity before synaptogenesis. By using neuronal whole mounts studied with transmission electron microscopy and GABA silver-enhanced immunogold staining, a quantitative comparison of growth cones after a day and a half in culture revealed that the growth cone of the longest proc...Continue Reading

References

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Citations

Mar 16, 2000·The Journal of Physiology·X B Gao, A N van den Pol
Dec 18, 2001·Journal of Neurobiology·T L DellovadeS A Tobet
Sep 6, 2000·The Journal of Comparative Neurology·M G Vannucchi, M S Faussone-Pellegrini
Nov 8, 2018·The European Journal of Neuroscience·Vania Carmona-AlcocerJennifer A Evans
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov
Jul 13, 1999·Journal of Neurophysiology·P R PatryloA Williamson

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