PMID: 8989386Oct 1, 1995Paper

GABA receptors precede glutamate receptors in hypothalamic development; differential regulation by astrocytes

Journal of Neurophysiology
G ChenA N van den Pol

Abstract

1. The developmental changes in gamma-aminobutyrate (GABA)-, glutamate-, and glycine-mediated currents in cultured embryonic neurons (n = 134) from rat hypothalamus were studied with the use of whole cell voltage-clamp recording. 2. GABA-evoked currents were detected in neurons cultured from 15-day embryos (E15) a few hours after plating. Every neuron studied from the time of plating at E15 to 2 wk later responded to GABA (30 microM). The peak and steady-state currents evoked by GABA increased by four- to fivefold within 2 wk in culture. The time constants of the desensitization of GABA currents did not change during this period. The properties of the responses to GABA were not altered by different culture densities or substrates. 3. Glycine activated receptors that were pharmacologically distinct from GABA receptors on hypothalamic neurons. The glycine responses increased by > 50-fold within 2 wk in culture. The percentage of cells responding to glycine (500 microM) was 20% at 0 days in vitro (DIV), and increased to 100% at 6 DIV. Astrocytes increased both the amplitude of glycine-mediated currents and the percentage of cells responding to glycine. 4. Glutamate-mediated currents developed later than GABA-mediated currents. The...Continue Reading

Citations

Aug 19, 1996·The Journal of Comparative Neurology·K Obrietan, A N van den Pol
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jennifer C Wilhelm, Peter Wenner
May 14, 2014·Metabolic Brain Disease·Connie Wu, Dandan Sun
Jun 10, 2015·Journal of Receptor and Signal Transduction Research·Colin G Rousseaux, Stephanie F Greene
Sep 3, 2002·Journal of Neurophysiology·Andrei B BelousovJanna V Denisova
Mar 16, 2000·The Journal of Physiology·X B Gao, A N van den Pol
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov
Jun 2, 2000·Molecular and Cellular Neurosciences·R ZhaiC C Garner
Jul 30, 2011·Progress in Neurobiology·Marissa S Kuzirian, Suzanne Paradis

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