GABA transport in Saccharomyces cerevisiae

Yeast
J McKelveyT G Cooper

Abstract

Gamma-aminobutyrate (GABA) accumulation in growing cultures of Saccharomyces cerevisiae was shown to occur by means of an active transport system that is inhibited by proton ionophores, azide, fluoride and arsenate ions. Transport occurred maximally at pH 5.0 and exhibited apparent Km values of 12 microM and 0.1 mM. Accumulated GABA did not efflux upon treatment with proton ionophores and exchanged with extracellular material only very slowly. However, release was complete upon treatment with nystatin. These observations raise the possibility that a major portion of intracellular GABA is sequestered in the vacuole. The response of GABA uptake to growth on various nitrogen sources suggested that uptake may be subject to several types of regulation.

References

Feb 14, 1977·Biochimica Et Biophysica Acta·M L GrethF Lacroute
Sep 1, 1977·Journal of Bacteriology·R Sumrada, T G Cooper
Dec 1, 1979·Journal of Bacteriology·V Turoscy, T G Cooper
Aug 1, 1978·Journal of Bacteriology·C A Zacharski, T G Cooper
Sep 1, 1979·Journal of Bacteriology·T G CooperR Sumrada
Feb 1, 1975·Journal of Bacteriology·T G Cooper, R Sumrada
Nov 2, 1972·Biochimica Et Biophysica Acta·A Kotyk, L Ríhová

Citations

Jul 21, 2009·Analytica Chimica Acta·Jang-Eun LeeYoung-Shick Hong
Jun 9, 2020·Journal of Applied Microbiology·G PerpetuiniR Tofalo
Oct 18, 2019·Microbiology and Molecular Biology Reviews : MMBR·Frans BianchiB Poolman
Jun 1, 1992·FEMS Microbiology Reviews·P J Large

Related Concepts

Gamma-Aminobutyric Acid, Zinc Salt (2:1)
Hydrogen-Ion Concentration
Nitrogen
Saccharomyces cerevisiae

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