Jul 1, 1997

GABA(A) receptor activation triggers a Cl- conductance increase and a K+ channel blockade in cerebellar granule cells

Neuroscience
C LabrakakisHelmut Kettenmann

Abstract

GABA(A) receptor activation in cerebellar granule cells induced a complex physiological response, namely the activation of a Cl- conductance in concert with a blockade of the resting K+ outward conductance (by 71% as compared to controls). Both responses were mediated by the activation of GABA(A) receptors, since they were both mimicked by the GABA(A) receptor agonist muscimol and antagonized by picrotoxin and bicuculline. A substantial decrease of the mean open time of single, outwardly rectifying K+ channels was triggered by GABA as revealed from cell-attached recordings; this finding implies that an intracellular pathway links GABA(A) receptors and K+ channels. Furthermore, this action of GABA is mediated through the cytoplasm, as experiments with the cell-attached patch-clamp technique show. GABA induced a prominent membrane depolarization ranging from 10 to 25 mV as revealed by current-clamp recordings of gramicidin (or nystatin) permeabilized patches, thus selecting conditions not to perturb the physiological Cl- gradient across the cell. Our findings imply that the GABA-activated Cl- current depolarized the membrane as described for immature neurons. The blockade of the resting K+ channel conductance acts in concert and ...Continue Reading

Mentioned in this Paper

Chloride Ion Level
Potassium Channel Blockers
Resting Potentials
Neurons
Picrotoxin
Gammalon
Barium
Voltage-Clamp Techniques
Calcium-Activated Chloride Channels
Muscimol

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