GABA(A) receptor activation triggers a Cl- conductance increase and a K+ channel blockade in cerebellar granule cells

Neuroscience
C LabrakakisH Kettenmann

Abstract

GABA(A) receptor activation in cerebellar granule cells induced a complex physiological response, namely the activation of a Cl- conductance in concert with a blockade of the resting K+ outward conductance (by 71% as compared to controls). Both responses were mediated by the activation of GABA(A) receptors, since they were both mimicked by the GABA(A) receptor agonist muscimol and antagonized by picrotoxin and bicuculline. A substantial decrease of the mean open time of single, outwardly rectifying K+ channels was triggered by GABA as revealed from cell-attached recordings; this finding implies that an intracellular pathway links GABA(A) receptors and K+ channels. Furthermore, this action of GABA is mediated through the cytoplasm, as experiments with the cell-attached patch-clamp technique show. GABA induced a prominent membrane depolarization ranging from 10 to 25 mV as revealed by current-clamp recordings of gramicidin (or nystatin) permeabilized patches, thus selecting conditions not to perturb the physiological Cl- gradient across the cell. Our findings imply that the GABA-activated Cl- current depolarized the membrane as described for immature neurons. The blockade of the resting K+ channel conductance acts in concert and ...Continue Reading

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Citations

Nov 13, 2001·The European Journal of Neuroscience·M SynowitzH Kettenmann
Sep 30, 1998·The European Journal of Neuroscience·C LabrakakisH Kettenmann
Jan 13, 2005·Acta Neurologica Scandinavica·B Schaller
May 25, 2005·Molecular and Cellular Neurosciences·Li-Ping WangHelmut Kettenmann
Jul 13, 1999·Journal of Neurophysiology·L K Bekar, W Walz

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