GABA(B) presynaptic receptors modulate glycine exocytosis from mouse spinal cord and hippocampus glycinergic nerve endings

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Cristina RomeiLuca Raiteri

Abstract

Glycine release and modulation of glycine exocytosis by presynaptic receptors have been rarely studied. We here investigate on the existence and the pharmacological profile of GABA(B) receptors regulating glycine release in mouse spinal cord and hippocampus. Synaptosomes were preincubated with [(3)H]glycine in presence of the glycine transporter 1 inhibitor NFPS, in order to study release from glycinergic terminals selectively labelled through the glycine transporter 2, and depolarized in superfusion with concentrations of KCl (12 and 15 mM) previously found to elicit exocytosis of glycine. Exposure of synaptosomes to (-)-baclofen caused inhibition of [(3)H]glycine overflow: EC(50)=0.62+/-0.08 microM, E(max) approximately 65% in the spinal cord and EC(50)=0.58+/-0.07 microM, E(max) approximately 55% in the hippocampus. The effect of 3 microM (-)-baclofen in the spinal cord was prevented by the GABA(B) receptor antagonists CGP 52432 (IC(50)=22.6+/-2.15 microM) and CGP 35348 (IC(50)=5.99+/-1.21 microM), whereas phaclofen was ineffective. In the hippocampus, the CGP antagonists were more potent than in the spinal cord, their IC(50) values amounting to 0.81+/-0.08 microM and to 0.94+/-0.09 microM, respectively; phaclofen (100-300 m...Continue Reading

References

Aug 18, 1995·Journal of Medicinal Chemistry·W FroestlH R Olpe
Aug 18, 1995·Journal of Medicinal Chemistry·W FroestlR Bernasconi
May 1, 1994·The Journal of Physiology·D B ReichlingA B MacDermott
Jul 1, 1993·Trends in Pharmacological Sciences·G Bonanno, M Raiteri
Dec 5, 1997·Brain Research. Molecular Brain Research·I PoyatosF Zafra
Jul 17, 1998·Science·P JonasJ Sandkühler
May 10, 2000·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·D C JavittC E Schroeder
Mar 30, 2001·The Journal of Biological Chemistry·A GeerlingsC Aragón
Jul 5, 2001·Cellular and Molecular Life Sciences : CMLS·P Legendre
Aug 10, 2001·Journal of Medicinal Chemistry·W L CaulfieldH Sundaram
Mar 29, 2002·The European Journal of Neuroscience·Cristina MarchettiAndrea Nistri
Feb 5, 2003·Neuro-Signals·A R CalverM Pangalos
Aug 19, 2003·Naunyn-Schmiedeberg's Archives of Pharmacology·O DudeckT J Feuerstein
Sep 29, 2004·Biochemical Pharmacology·Andrés CouveMenelas N Pangalos
Nov 24, 2004·Molecular and Cellular Neurosciences·Lydia DanglotAlain Bessis
Mar 8, 2005·Cerebral Cortex·Beatriz CubelosFrancisco Zafra
Mar 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin K YeeDetlev Boison
May 23, 2006·Pharmacological Reviews·Maurizio Raiteri
Jun 15, 2006·Cellular and Molecular Neurobiology·Joseph T Coyle
Jun 30, 2006·Journal of Neurochemistry·Heinrich Betz, Bodo Laube
Apr 17, 2007·Current Opinion in Neurobiology·Daniel Ulrich, Bernhard Bettler
Nov 13, 2007·Brain Research·Anna NilssonErik Sundström
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine

❮ Previous
Next ❯

Citations

Feb 9, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Cristina RomeiLuca Raiteri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-N-Methyl-D-Aspartate Receptor Encephalitis

Anti-N-Methyl-D-Aspartate (NMDA) receptor encephalitis is a form of of brain inflammation due to antibodies against NMDA receptors. Discover the latest research on anti-NMDA receptor encephalitis here.