GABAB receptor-mediated inhibition of spontaneous inhibitory synaptic currents in rat midbrain culture

The Journal of Physiology
J RohrbacherU Misgeld

Abstract

1. Tight-seal, whole-cell recording was used to study GABAB receptor-mediated inhibition of spontaneous inhibitory synaptic currents in cultured rat midbrain neurones. 2. Spontaneous miniature inhibitory postsynaptic currents (mIPSCs) were recorded in tetrodotoxin (TTX), Cd2+ and Ba2+. (R)-(-)-baclofen reduced the frequency of mIPSCs through a presynaptic mechanism. The EC50 for this effect was 7 microM. It was antagonized by the GABAB receptor antagonist CGP55845A (0.5 microM). 3. In pertussis toxin (PTX)-treated cultures, some GABAB receptor-mediated reduction of the frequency of mIPSCs persisted. In contrast, PTX treatment totally abolished inhibition of miniature excitatory postsynaptic currents (mEPSCs). 4. In PTX-treated cultures, a saturating concentration of (R)-(-)-baclofen inhibited action potential-generated IPSCs but no EPSCs. 5. PTX treatment abolished the (R)-(-)-baclofen-mediated inhibition of high voltage-activated somatic Ca2+ currents and of spontaneous IPSCs depending on presynaptic Ca2+ entry. 6. We conclude that cellular mechanisms underlying GABAB receptor-mediated inhibition of mIPSCs contribute to auto-inhibition of GABA release.

Citations

Sep 1, 2004·Cell and Tissue Research·Ulrich Misgeld
Aug 16, 2002·The Journal of Physiology·Zafiris J DaskalakisRobert Chen
Apr 22, 2008·Alcoholism, Clinical and Experimental Research·Jonathan W TheileRichard A Morrisett
Jul 23, 2004·Physiological Reviews·Bernhard BettlerMartin Gassmann
Jun 10, 2005·Journal of Neurophysiology·Michela GiustizieriNicola Berretta
Dec 10, 2009·Neuroreport·Masatoshi TakitaMichiko Ohtomi
Sep 19, 2003·Journal of Neurophysiology·Nikolai AxmacherRaphael Ritz
Feb 15, 2001·The Journal of Comparative Neurology·Y GoncharA Burkhalter

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