GABA(B) receptor-mediated modulation of hypocretin/orexin neurones in mouse hypothalamus.

The Journal of Physiology
Xinmin XieThomas S Kilduff

Abstract

Hypocretin/orexin (Hcrt) is a critical neurotransmitter for the maintenance of wakefulness and has been implicated in several other functions, including energy metabolism and reward. Using whole-cell patch-clamp recordings from transgenic mice in which enhanced green fluorescent protein was linked to the Hcrt promoter, we investigated GABAergic control of the Hcrt neurones in hypothalamic slices. Bath application of GABA or muscimol caused an early hyperpolarization mediated by Cl(-) and a late depolarization mediated by the efflux of bicarbonate. These GABA(A) receptor-mediated responses were blocked by picrotoxin and bicuculline. Under the GABA(A) blockade condition, GABA produced consistent hyperpolarization, decreased firing rate and input resistance. The selective GABA(B) agonist (R)-baclofen caused a similar response with an EC(50) of 7.1 mum. The effects of (R)-baclofen were blocked by the GABA(B) antagonist CGP 52432 but persisted in the presence of tetrodotoxin, suggesting direct postsynaptic effects. The existence of GABA(B) modulation was supported by GABA(B(1)) subunit immunoreactivity on Hcrt cells colabelled with antisera to the Hcrt-2 peptide. Furthermore, GABA(B) receptor activation inhibited the presynaptic rel...Continue Reading

References

Mar 3, 1992·European Journal of Pharmacology·X Xie, T G Smart
May 1, 1991·Synapse·N B MercuriG Bernardi
Jan 1, 1974·Archives of Neurology·C GuilleminaultW C Dement
Feb 1, 1995·The European Respiratory Journal·A J FinnimoreR D McEvoy
May 19, 1994·Neuroscience Letters·G JuhászV Crunelli
Jul 15, 1996·The Journal of Physiology·G ChenA N van den Pol
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·L de LeceaJ G Sutcliffe
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y DateM Nakazato
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·K E Vogt, R A Nicoll
Apr 2, 1999·The European Journal of Neuroscience·D R StevensH L Haas
Jan 1, 2000·Lancet·S NishinoE Mignot
Jul 25, 2000·Trends in Neurosciences·T S Kilduff, C Peyron
Oct 31, 2000·Neuron·T C ThannickalJ M Siegel
Feb 13, 2001·Progress in Neurobiology·R E BrownH L Haas
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A ManfridiM Mancia
Nov 24, 2001·Trends in Neurosciences·C B SaperT E Scammell
Nov 28, 2001·The European Journal of Neuroscience·L BayerM Mühlethaler
Dec 6, 2001·Neuroscience·E EggermannM Mühlethaler
Jan 29, 2002·Biochemical and Biophysical Research Communications·Akihiro YamanakaTakeshi Sakurai
Feb 11, 2003·Neuron·Valentin Stein, Roger A Nicoll
Mar 21, 2003·Biochemical and Biophysical Research Communications·Akihiro YamanakaTakeshi Sakurai
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Barbara E Jones
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Dennis McGinty, Ronald Szymusiak
Apr 9, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Min WuMeenakshi Alreja

❮ Previous
Next ❯

Citations

Feb 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Taizo MatsukiTakeshi Sakurai
Jul 20, 2012·Physiological Reviews·Ritchie E BrownRobert W McCarley
Jan 26, 2013·Stroke; a Journal of Cerebral Circulation·Xiaoxing XiongXinmin Simon Xie
Mar 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Junko HaraThomas S Kilduff
Sep 12, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yan RaoXiao-Bing Gao
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peter BergmanTomas Hökfelt
Feb 29, 2016·Pharmacological Reports : PR·Anil KumarSupriti Choudhary
May 14, 2011·Trends in Pharmacological Sciences·Takeshi Sakurai, Michihiro Mieda
Aug 6, 2009·Progress in Neurobiology·Mortimer Mamelak
Oct 4, 2007·Frontiers in Neuroendocrinology·Kousaku Ohno, Takeshi Sakurai
Jul 6, 2010·The European Journal of Neuroscience·Oum Kaltoum HassaniBarbara E Jones
Dec 20, 2013·Neuromodulation : Journal of the International Neuromodulation Society·Konstantinos MargetisDamianos E Sakas
Mar 25, 2010·Alcoholism, Clinical and Experimental Research·Irene MorgansternSarah F Leibowitz
Jul 17, 2010·Annals of the New York Academy of Sciences·Takeshi SakuraiNatsuko Tsujino
Nov 26, 2015·The Psychiatric Clinics of North America·Michael D Schwartz, Thomas S Kilduff
May 28, 2013·Behavioural Brain Research·Tomomi TsunematsuAkihiro Yamanaka
Jun 5, 2012·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Matthias HochJasper Dingemanse
Oct 9, 2012·Neuron·Olivier Civelli
Mar 6, 2015·Frontiers in Systems Neuroscience·Juliette A BrownGina M Leinninger
Dec 21, 2016·The Journal of Comparative Neurology·Lindsay J AgostinelliThomas E Scammell
Feb 15, 2007·Nature Reviews. Neuroscience·Takeshi Sakurai
Jun 24, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Julia BurtMichiru Hirasawa
Oct 5, 2012·American Journal of Physiology. Cell Physiology·Jyrki P Kukkonen
Jun 25, 2009·Pharmacological Reviews·Natsuko Tsujino, Takeshi Sakurai
Jun 18, 2017·Frontiers in Physiology·Sergio ChieffiAntonietta Messina
Jun 22, 2018·Frontiers in Neurology·Susan M TyreeLuis de Lecea
May 31, 2014·Journal of Clinical Psychopharmacology·Konstantinos MargetisDamianos Sakas
Mar 16, 2018·Frontiers in Neurology·Antonietta MessinaMarco Carotenuto
Oct 28, 2019·Diabetes/metabolism Research and Reviews·Ernest AdeghateSaif Abdo Qahtan
Jul 28, 2020·Journal of Neural Transmission·Raffaele NardoneViviana Versace
Apr 21, 2017·Current Opinion in Neurobiology·Takayuki Yamashita, Akihiro Yamanaka
May 21, 2017·Neuroscience Research·Daisuke Ono, Akihiro Yamanaka
Nov 19, 2021·Endocrine Reviews·Xiao-Bing Gao, Tamas L Horvath

❮ Previous
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