GABAergic modulation of motor-driven behaviors in juvenile Drosophila and evidence for a nonbehavioral role for GABA transport

Journal of Neurobiology
Sandra M LealW S Neckameyer

Abstract

We have identified specific GABAergic-modulated behaviors in the juvenile stage of the fruit fly, Drosophila melanogaster via systemic treatment of second instar larvae with the potent GABA transport inhibitor DL-2,4-diaminobutyric acid (DABA). DABA significantly inhibited motor-controlled body wall and mouth hook contractions and impaired rollover activity and contractile responses to touch stimulation. The perturbations in locomotion and rollover activity were reminiscent of corresponding DABA-induced deficits in locomotion and the righting reflex observed in adult flies. The effects were specific to these motor-controlled behaviors, because DABA-treated larvae responded normally in olfaction and phototaxis assays. Recovery of these behaviors was achieved by cotreatment with the vertebrate GABA(A) receptor antagonist picrotoxin. Pharmacological studies performed in vitro with plasma membrane vesicles isolated from second instar larval tissues verified the presence of high-affinity, saturable GABA uptake mechanisms. GABA uptake was also detected in plasma membrane vesicles isolated from behaviorally quiescent stages. Competitive inhibition studies of [3H]-GABA uptake into plasma membrane vesicles from larval and pupal tissues ...Continue Reading

References

Mar 1, 1991·Neurochemical Research·D B SattelleJ J Rauh
Jun 1, 1991·Journal of Neurobiology·M G GorczycaC F Wu
Mar 1, 1990·Journal of Neurochemistry·F R JacksonS J Kulkarni
Feb 1, 1990·Genetics·M Lilly, J Carlson
Mar 24, 1987·Brain Research·H Bülthoff, I Bülthoff
Aug 1, 1984·Neuropharmacology·G M StrainT A Tucker
Jun 18, 1980·Molecular and Cellular Biochemistry·P Krogsgaard-Larsen
May 6, 1983·European Journal of Pharmacology·M J CroucherP Krogsgaard-Larsen
Apr 20, 1995·Archives of Biochemistry and Biophysics·D MbunguS S Gill
Jul 22, 1993·Nature·S L McIntireH R Horvitz
Jul 22, 1993·Nature·S L McIntireH R Horvitz
Oct 1, 1996·Journal of Neurochemistry·H FukuraM Igarashi
Oct 1, 1996·Neurochemistry International·L A Borden
Dec 1, 1996·British Journal of Pharmacology·A M Hosie, D B Sattelle
Jan 1, 1995·Invertebrate Neuroscience : in·K Aronstein, R Ffrench-Constant
Sep 1, 1996·Invertebrate Neuroscience : in·K AronsteinR Ffrench-Constant
Oct 13, 1998·Life Sciences·I S Shiah, L N Yatham
Oct 28, 1998·Physiological Reviews·M PalacínA Zorzano
Jan 5, 1999·The Journal of Biological Chemistry·E M Bernstein, M W Quick
Mar 17, 1999·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·J R MartinM Heisenberg
Apr 23, 1999·Invertebrate Neuroscience : in·W S Neckameyer, R L Cooper
Nov 26, 1999·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·J R MartinM Heisenberg
Jan 29, 2000·The European Journal of Neuroscience·S Fellippa-MarquesF Clarac
Sep 9, 2000·Journal of Neurophysiology·T G DeliaginaY I Arshavsky
Oct 4, 2000·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·F J EisenhartW B Kristan
Feb 13, 2001·The European Journal of Neuroscience·M MezlerK Raming

❮ Previous
Next ❯

Citations

Aug 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Akihiko YamamotoRobert R H Anholt
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jamey S KainBenjamin L de Bivort
Oct 19, 2013·Nucleic Acids Research·Xianghua LiMary A O'Connell
Aug 30, 2008·Neurobiology of Learning and Memory·Abdessalam Kacimi El HassaniCatherine Armengaud
Jan 2, 2021·Frontiers in Behavioral Neuroscience·Maggie M ChvilicekAdrian Rothenfluh
Jun 22, 2010·Toxicon : Official Journal of the International Society on Toxinology·S A BanackP A Cox

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Sung-Tae HongJaeseob Kim
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Tong LiuAike Guo
Proceedings of the National Academy of Sciences of the United States of America
Divya SitaramanTroy Zars
© 2021 Meta ULC. All rights reserved