GABAergic neurons in the embryonic olfactory pit/vomeronasal organ: maintenance of functional GABAergic synapses in olfactory explants

Developmental Biology
S WrayH Gainer

Abstract

In previous work, we showed a robust gamma-aminobutyric acid (GABAergic) synaptic input onto embryonic luteinizing hormone-releasing hormone (LHRH) neurons maintained in olfactory explants. In this study, we identify GABAergic neurons in olfactory pit (OP) of embryonic mice in vivo and study, using patch-pipet whole-cell current and voltage clamp techniques, synaptic interactions of these neurons in explant cultures. In vivo, glutamate decarboxylase (GAD, the enzyme which synthesizes GABA) mRNA was first detected in nasal regions on Embryonic Day (E) 11.5. From E12.5 to E13.5, robust GAD expression was localized to cells primarily in the ventral aspect of the OP. GAD mRNA was not detected over dorsally located cells in olfactory sensory or respiratory epithelium. In addition, GAD mRNA was not observed in cells along olfactory axons. GAD mRNA was dramatically reduced in the OP/vomeronasal organ by E16.5. Using antibodies against both GABA and GAD, immunopositive axonal-like tracts were detected in the nasal septum on E12.5. GABAergic staining decreased by E13.5. To examine synaptic interactions of these GABAergic cells, embryonic olfactory explants were generated and maintained in serum-free media. As explants spread, neuron-lik...Continue Reading

Citations

Oct 19, 2002·Behavioural Brain Research·Anna Maria AloisiAndrea Scaramuzzino
Sep 3, 2003·Progress in Neurobiology·Mimi Halpern, Alino Martínez-Marcos
Dec 12, 2001·Molecular and Cellular Endocrinology·S A TobetG A Schwarting
Oct 13, 2000·The European Journal of Neuroscience·S X SimonianA E Herbison
Jun 18, 2010·Endocrinology·Stephanie ConstantinSusan Wray
Sep 15, 2012·Journal of Cell Science·Filippo CasoniSusan Wray
Dec 17, 2014·Frontiers in Neuroscience·Miho WatanabeJunichi Nabekura
Feb 21, 2006·International Review of Cytology·Martin Witt, Thomas Hummel
Jul 20, 2002·Frontiers in Neuroendocrinology·S Wray
Jul 22, 2004·Journal of Neurobiology·Catherine A Priest, Adam C Puche
Dec 21, 2017·Reproductive Medicine and Biology·Haruhiko KanasakiSatoru Kyo
Jul 9, 2009·Physiological Reviews·Roberto TirindelliAnna Menini
Jan 18, 2006·The Journal of Pharmacology and Experimental Therapeutics·Rafal M KaminskiMichael A Rogawski
Mar 5, 2016·Endocrinology·Ulrike KlenkeSusan Wray
Aug 26, 1998·Microscopy Research and Technique·M GarrosaF J Esteban
Jul 30, 2019·Frontiers in Cell and Developmental Biology·Hyun-Ju ChoSusan Wray
Oct 26, 1999·Science·E B Keverne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.