GABARAP is not essential for GABA receptor targeting to the synapse

The European Journal of Neuroscience
G A O'SullivanH Betz

Abstract

GABA(A) receptors (GABA(A)Rs) containing the gamma2 subunit are thought to require the interacting protein GABARAP (GABA(A)R associated protein) for trafficking to the neuronal plasma membrane. In order to assess whether GABARAP is required for GABA(A) receptor accumulation at synaptic sites, we analysed a GABARAP knockout mouse. GABARAP deficient mice are phenotypically normal and do not show up-regulation of other GABARAP homologues. Also, the total number of GABA(A)Rs, as assessed by benzodiazepine binding, is unaffected by the loss of GABARAP. Immunocytochemistry of cortical sections showed no differences in the expression and punctate distribution of the gamma2 subunit and the receptor anchoring protein gephyrin between GABARAP deficient and wild-type mice. Thus, GABARAP is not essential for trafficking gamma2 subunit containing GABA(A)Rs to the neuronal plasma membrane or targeting them to inhibitory synapses.

References

Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F PfeifferH Betz
Jan 5, 1996·The Journal of Biological Chemistry·C N ConnollyStephen J Moss
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·Matthias KneusselH Betz
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·L ChenRichard W Olsen
Jun 21, 2001·Molecular and Cellular Neurosciences·Matthias KneusselH Betz
Jan 31, 2002·EMBO Reports·Vassiliy N BavroWinfried Weissenhorn
May 9, 2002·FEBS Letters·Frank GreenCaroline A Enns
Jun 28, 2002·Brain Research. Brain Research Reviews·Matthias Kneussel
Nov 20, 2003·Brain Research. Molecular Brain Research·Christophe NemosRégis Delage-Mourroux
Mar 10, 2004·The Journal of Biological Chemistry·Andrea B EverittPeter W Gage
Nov 9, 2004·Biochemical and Biophysical Research Communications·Virginie MansuyRégis Delage-Mourroux
Dec 17, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tarek A LeilRichard W Olsen

Citations

Jul 29, 2010·Urological Research·Nati HernandoHeini Murer
Apr 1, 2010·Pflügers Archiv : European journal of physiology·Sonja C ReiningNati Hernando
Sep 3, 2010·Nature Cell Biology·Noboru Mizushima, Beth Levine
Apr 3, 2008·Nature Reviews. Neuroscience·Tija C JacobRachel Jurd
Nov 13, 2008·The Journal of Biological Chemistry·Yong ChenLee-Yuan Liu-Chen
Nov 4, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emile G BruneauMohammed Akaaboune
Feb 20, 2009·American Journal of Physiology. Renal Physiology·Sonja C ReiningNati Hernando
Jan 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wei Chou TsengVann Bennett
Aug 14, 2013·Autophagy·Kim Wager, Claire Russell
Apr 18, 2015·Experimental & Molecular Medicine·Gayoung Choii, Jaewon Ko
Nov 7, 2006·Journal of Neurochemistry·Zi-Wei Chen, Richard W Olsen
Feb 21, 2014·Nature Reviews. Neuroscience·Shiva K Tyagarajan, Jean-Marc Fritschy
May 17, 2007·Biology of the Cell·Matthias Kneussel, Sven Loebrich
Aug 19, 2017·Autophagy·Akiko KumaNoboru Mizushima
Aug 14, 2009·The FEBS Journal·Jeannine MohrlüderDieter Willbold
Sep 8, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marco B E SchaafKasper M A Rouschop
Aug 1, 2020·The Journal of Biological Chemistry·Nathan NguyenThomas J Melia
Feb 22, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhirong ZhangJiahuai Han
Sep 14, 2017·Developmental Neurobiology·Joshua M Lorenz-Guertin, Tija C Jacob
Sep 8, 2018·Frontiers in Cellular Neuroscience·Joshua M Lorenz-GuertinTija C Jacob
Apr 10, 2020·Neuronal Signaling·Fumi Suomi, Thomas G McWilliams
Dec 10, 2016·Frontiers in Oncology·Tom G KeulersKasper M A Rouschop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.