GABARAPL1 acts as a potential marker and promotes tumor proliferation and metastasis in triple negative breast cancer

Oncotarget
Li RanGebo Wen

Abstract

GABAA-receptor-associated protein like-1 (GABARAPL1) is involved in a variety of cancers. The purpose of this study was to investigate the expression, prognostic roles and functions of GABARAPL1 in triple negative breast cancer (TNBC). Quantitative real-time PCR (qRT-PCR) showed that GABARAPL1 was up regulated in both TNBC cell lines and clinical TNBC tissues. High GABARAPL1 expression level was associated with shorter overall survival (OS) and disease free survival (DFS). Furthermore, inhibition of GABARAPL1 suppressed cell proliferation, tumorigenesis, invasion and metastasis, and induced cell apoptosis. We found that metadherin (MTDH) was a downstream target of GABARAPL1. Inhibition of GABARAPL1 suppressed the mRNA and protein expression of MTDH, and overexpression of MTDH could reverse the effects of GABARAPL1 inhibition, which meant GABARAPL1 performed its function partly through MTDH. Our findings demonstrate that GABARAPL1 acts as a tumor promoter in TNBC partly through MTDH. Targeting at GABARAPL1 could be a potential therapeutic strategy for TNBC.

References

Apr 21, 2010·Autophagy·Fatima Zahra ChakramaMichaël Boyer-Guittaut
Jun 22, 2010·Nature·Christian BehrendsJ Wade Harper
Jul 20, 2010·Biochimica Et Biophysica Acta·Suning ChenJian Zhang
Sep 3, 2010·The Journal of Biological Chemistry·Sixin JiangPeter J Roach
Dec 17, 2010·The Journal of Biological Chemistry·Arunima SenguptaKatherine E Yutzey
May 21, 2011·Autophagy·Jaclyn Nicole Le GrandMichaël Boyer-Guittaut
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yanquan ZhangZhijie Chang
Oct 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kai ZhuJian Zhou
Aug 21, 2012·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Eriko TokunagaYoshihiko Maehara
Oct 4, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·G von Minckwitz, M Martin
Oct 4, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J CrownG Gullo
Oct 4, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P Boyle
Jan 31, 2013·Molecular Cancer Research : MCR·Dawn M Kochanek, David G Wells
Jul 31, 2013·Advances in Cancer Research·Devanand Sarkar
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Johanna I KiiskiHeli Nevanlinna
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Jun 8, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chong-Wei XieWei Zhang
Jul 15, 2015·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Tom G KeulersKasper M A Rouschop
Sep 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fangjuan DuMaowu Yang
Oct 18, 2015·BMC Cancer·Eric HervouetMichaël Boyer-Guittaut
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
xenograft
flow cytometry
electrophoresis

Software Mentioned

SPSS16

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Breast Cancer Triple-N

Breast cancer cells have receptors for estrogen, progesterone, HER2 receptors (also called ERBB2). Triple-negative breast cancers do not have any of these receptors. Here are the latest discoveries pertaining to triple-negative breast cancers.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.