GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis

FEBS Letters
Alessandra DonadiniStefano Biffo

Abstract

Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

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Citations

Jul 12, 2017·Oncology Letters·Wei ZhuXing Yan Liu
Jul 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Thomas C J TanRose Zamoyska

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