GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis
Abstract
Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.
References
Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells.
Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6
Citations
Eukaryotic initiation factor 6 mediates a continuum between 60S ribosome biogenesis and translation.
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