Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death.

Bioelectrochemistry
Franck M AndreAndrei G Pakhomov

Abstract

It has been widely accepted that nanosecond electric pulses (nsEP) are distinguished from micro- and millisecond duration pulses by their ability to cause intracellular effects and cell death with reduced effects on the cell plasma membrane. However, we found that nsEP-induced cell death is most likely mediated by the plasma membrane disruption. We showed that nsEP can cause long-lasting (minutes) increase in plasma membrane electrical conductance and disrupt electrolyte balance, followed by water uptake, cell swelling and blebbing. These effects of plasma membrane permeabilization could be blocked by Gd(3+) in a dose-dependent manner, with a threshold at sub-micromolar concentrations. Consequently, Gd(3+) protected cells from nsEP-induced cell death, thereby pointing to plasma membrane permeabilization as a likely primary mechanism of lethal cell damage.

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Citations

Nov 26, 2010·The Journal of Membrane Biology·Oksana DyachokTerence F McDonald
May 29, 2012·The Journal of Membrane Biology·Mikhail A Rassokhin, Andrei G Pakhomov
Jun 13, 2012·Pigment Cell & Melanoma Research·Richard NuccitelliEdward C De Fabo
Jul 31, 2013·PloS One·Olga N PakhomovaAndrei G Pakhomov
Jul 10, 2012·Biochemical and Biophysical Research Communications·Richard NuccitelliJean Y Tang
Oct 10, 2015·Scientific Reports·Caleb C RothRandolph D Glickman
Feb 1, 2013·Cell Biology International·Changjiang Ding, Liaofu Luo
Nov 30, 2012·IEEE Transactions on Bio-medical Engineering·Yu-Hsuan WuP Thomas Vernier
Mar 19, 2013·Biochimica Et Biophysica Acta·Stefania RomeoP Thomas Vernier
Apr 12, 2015·Biophysical Journal·Lars H WegnerAude Silve
Jan 15, 2015·Biochimica Et Biophysica Acta·Andrei G PakhomovOlga N Pakhomova
Sep 10, 2014·Bioelectrochemistry·Gale L CravisoP Thomas Vernier
Aug 3, 2016·Biochemistry and Biophysics Reports·Iurii SemenovAndrei G Pakhomov
Jul 21, 2016·The Journal of Membrane Biology·Esin B SözerP Thomas Vernier
Aug 25, 2016·Biochemical and Biophysical Research Communications·Zachary A SteelmanBennett L Ibey
Jan 25, 2019·Scientific Reports·Alessandra RossiClaudia Muratori
Mar 1, 2017·The Journal of Membrane Biology·Claudia MuratoriOlga N Pakhomova
May 24, 2018·BioMed Research International·Tarique R BagalkotGale L Craviso
Feb 25, 2015·Archives of Biochemistry and Biophysics·Elena C Gianulis, Andrei G Pakhomov

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