PMID: 9557721Apr 29, 1998Paper

gag, vif, and nef genes contribute to the homologous viral interference induced by a nonproducer human immunodeficiency virus type 1 (HIV-1) variant: identification of novel HIV-1-inhibiting viral protein mutants

Journal of Virology
P D'AlojaM Federico

Abstract

We previously demonstrated that expression of the nonproducer F12-human immunodeficiency virus type 1 (HIV-1) variant induces a block in the replication of superinfecting HIV that does not depend on the down-regulation of CD4 HIV receptors. In order to individuate the gene(s) involved in F12-HIV-induced interference, vectors expressing each of the nine F12-HIV proteins were transfected in HIV-susceptible HeLa CD4 cells. Pools of cell clones stably producing each viral protein were infected with HIV-1, and virus release was measured in terms of reverse transcriptase activity in supernatants. We hereby demonstrate that HeLa CD4 cells expressing the F12-HIV gag, vif, or nef gene were resistant, to different degrees, to infection with T-cell-line-adapted HIV-1 strains. Conversely, expression of either the tat, rev, or vpu F12-HIV gene increased the rate of HIV release, and no apparent effects on HIV replication were observed in cells expressing either the F12-HIV vpr, pol, or env gene. No variation of CD4 exposure was detected in any of the uninfected HeLa CD4 pools. These data indicate that F12-HIV homologous viral interference is the consequence of the synergistic anti-HIV effects of Gag, Vif, and Nef proteins. Retrovirus vectors...Continue Reading

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Citations

Oct 6, 2000·Gene Therapy·K Frimpong, S A Spector
Feb 22, 2005·The Journal of General Virology·Shinya Omoto, Yoichi R Fujii
Mar 10, 2009·Biochemistry. Biokhimii︠a︡·L F Lideman, R A Gibadulin
Dec 1, 2009·Journal of Translational Medicine·Samuele E BurasteroLucia Lopalco
Jan 9, 2003·Microbiology and Immunology·Takuya YamamotoYoichi R Fuji
May 13, 2004·Journal of Virology·Luciana J CostaB Matija Peterlin
Mar 12, 1999·Journal of Virology·J H SimonM H Malim
Aug 13, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Silvia PerettiMaurizio Federico
Aug 19, 2005·Retrovirology·Micha NetheAntoinette C van der Kuyl

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