Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays

Journal of Experimental Botany
Yisen LiuJiehua Wang

Abstract

Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthesized each dodecapeptide and analysed itsi n vitro bioactivity on Arabidopsis seedlings. Similarly, but to a different extent, three types of poplar CLV3-related peptides caused root meristem consumption, phyllotaxis disorder, anthocyanin accumulation and failure to enter the bolting stage. In comparison, application of two poplar TDIF-related peptides led to root length promotion in a dose-dependent manner with an even stronger effect observed for poplar TDIF-like peptide than TDIF. Next, we constructed CaMV35S:PtCLE transgenic plants for each of the nine PtCLE genes. Phenotypic abnormalities exemplified by arrested shoot apical meristem and abnormal flower structure were found to be more dominant and severe in 35S:PtCLV3 and 35S:PtCLV3-like2 lines than in the 35S:PtCLV3-like line. Disordered vasculature was detected in both stem and hypocotyl cross-sections in Arabidopsis plants over-expressing poplar TDIF-related...Continue Reading

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Citations

May 28, 2016·Journal of Experimental Botany·Yasuka L YamaguchiShinichiro Sawa
May 28, 2016·Plant Signaling & Behavior·Zhijun LiuGuodong Wang
Jun 15, 2019·Plant Biotechnology Journal·Yingying ZhuLaigeng Li
Mar 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Yawen SongJiehua Wang

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