Nov 18, 2020Paper

Gain-of-function assay for SARS-CoV-2 M pro inhibition in living cells

BioRxiv : the Preprint Server for Biology
S. A. MoghadasiReuben Harris

Abstract

The main protease, M pro , of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M pro function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.

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