Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment

Development
Anastasia EskovaUwe Irion

Abstract

The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. Many of the known mutations that alter the pattern act cell-autonomously in pigment cells, and our knowledge about external regulators is limited. Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that lead to broken stripes and short fins. A loss-of-function aqp3a allele, generated by CRISPR-Cas9, has no phenotypic consequences, demonstrating that Aqp3a is dispensable for normal development. Strikingly, the pigment cells from dominant mau mutants are capable of forming a wild-type pattern when developing in a wild-type environment, but the surrounding tissues in the mutants influence pigment cell behaviour and interfere with the patterning process. The mutated amino acid residues in the dominant alleles line the pore surface of Aqp3a and influence pore permeability. These results demonstrate an important effect of the tissue environment on pigment cell behaviour and, thereby, on pattern formation.

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Citations

Apr 17, 2018·International Journal of Molecular Sciences·Lanmei WangJianjun Fu
Oct 27, 2018·Science·Hugo F Gante
Apr 24, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Matthew P HarrisJennifer Lanni
May 30, 2019·ELife·Lauren M SaundersDavid M Parichy
Jul 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mikhail InyushinAstrid Zayas-Santiago
Oct 23, 2019·Frontiers in Endocrinology·Johannes R SchmidtFranziska Knopf
Jul 1, 2020·Current Opinion in Genetics & Development·Alexandria Volkening

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Methods Mentioned

BETA
Transgenic
protein folding
in vitro transcription
transfection
PCR

Software Mentioned

GraphPad
Modeller
ZEN
Fiji
HHpred
InStat

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