Gain of imprinting of SLC22A18 sense and antisense transcripts in human breast cancer

Genomics
E GallagherA Mc Cann

Abstract

The 11p15.5 region harbors three imprinted sense/antisense transcript pairs, SLC22A18/SLC22A18AS, IGF2/IGF2AS (PEG8), and KCNQ1/KCNQ1OT1 (LIT1). SLC22A18 (solute carrier family 22 (organic cation transporter) member 18) and its antisense transcript SLC22A18AS are paternally suppressed in fetal samples. In adult tissue, SLC22A18 displays polymorphic imprinting, but the imprinting status of SLC22A18AS remains elusive. SLC22AI8 DNA-PCR-RFLP analysis using NlaIII restriction digestion identified SLC22A18 heterozygotes within this breast tissue cohort (n = 89). Commercial sequencing identified informative SLC22A18AS samples. Random hexamer-primed cDNA synthesis, SLC22A18/SLC22A18AS-specific PCR, and imprinting evaluation by commercial sequencing demonstrated that SLC22A18AS displays a nonimprinted profile in reduction mastectomies (n = 6). However, SLC22A18 showed a gain of imprinting (GOI) in 1/4 of these normal cases. In the malignant cohort, GOI was also demonstrated in 18% for SLC22A18 and 14% for SLC22A18AS, occurring concomitantly in one case. This study reports the imprinting status of SLC22A18AS in adult tissue, and shows that GOI affects both the sense, and antisense transcripts at this locus in human breast tissue.

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Citations

Aug 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shaw-Hwa LoTian Zheng
Mar 23, 2011·Nucleic Acids Research·Aiping ZhangRandy L Jirtle
Jun 10, 2006·Annales de chirurgie·G ChampaultUNKNOWN Société Française de Chirurgie Endoscopique
Dec 11, 2007·Biochimica Et Biophysica Acta·Barbara T McGroganAmanda McCann
Mar 22, 2013·Journal of Translational Medicine·Sheng-Hua ChuPu-Cha Jiang
Oct 16, 2012·American Journal of Respiratory and Critical Care Medicine·Emily S WanDawn L DeMeo
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michelle E ArmstrongSeamas C Donnelly
Dec 18, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Muhammad A AlviRebecca C Fitzgerald
Apr 15, 2010·Cancer Research·Angelo VeroneseMassimo Negrini

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