PMID: 2107541Mar 1, 1990Paper

GAL4 transcription factor is not a "zinc finger" but forms a Zn(II)2Cys6 binuclear cluster

Proceedings of the National Academy of Sciences of the United States of America
T Pan, J E Coleman

Abstract

The DNA-binding domain of the transcription factor GAL4, consisting of the 62 N-terminal residues and denoted GAL4(62*), contains a Cys-Xaa2-Cys-Xaa6-Cys-Xaa6-Cys-Xaa2-Cys-Xaa6+ ++-Cys motif, which has been shown previously to bind two Zn(II) or Cd(II) ions. Binding of Zn(II) or Cd(II) is essential for the recognition by GAL4 of the specific palindromic DNA sequence to which it binds upstream of genes for galactose-metabolizing enzymes, the UASG sequence. On the basis of the 113Cd NMR chemical shifts of the two bound 113Cd(II) ions, we propose a binuclear cluster model for this Zn(II)-binding subdomain. 1H-113Cd heteronuclear multiple-quantum NMR spectroscopy and phase-sensitive double-quantum filtered 1H correlation spectroscopy of the 112Cd(II)- and 113Cd(II)-substituted GAL4(62*) derivatives provide direct evidence that the two bound 113Cd(II) ions are coordinated only by the six cysteine residues, two of which form bridging ligands between the two 113Cd(II) ions. The latter can be identified from the pattern of 1H-113Cd J coupling. Thus a binuclear metal ion cluster rather than a "zinc finger" is formed by the six cysteine residues of the GAL4 DNA-binding domain. This model can be directly applied to eight other fungal tran...Continue Reading

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T Pan, J E Coleman
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Johnston, J Dover

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Citations

May 1, 1992·Protein Science : a Publication of the Protein Society·L A Basile, J E Coleman
Apr 1, 1994·Protein Science : a Publication of the Protein Society·K K Rodgers, J E Coleman
Apr 9, 2013·Applied Microbiology and Biotechnology·Perng-Kuang Chang, Kenneth C Ehrlich
Dec 1, 1992·The International Journal of Biochemistry·W M Krajewska
Jun 7, 1996·Biochimica Et Biophysica Acta·M Akiyama, H Nakashima
Aug 1, 1991·Current Opinion in Biotechnology·W J Chazin
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Oct 30, 1998·Cellular Signalling·W S Liu, C A Heckman
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert A MoxleySuchareeta Mitra
Jan 12, 1999·The Biochemical Journal·J DongS H Lee
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S MalikR G Roeder
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B L ValleeD S Auld
Mar 1, 1995·The Journal of Cell Biology·A V StrunnikovD Koshland
Aug 13, 2011·Molecular Plant-microbe Interactions : MPMI·Chunzhao ZhaoDingzhong Tang
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Mar 22, 2014·BMC Genomics·Richard B ToddRonald P de Vries
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Apr 28, 2007·Fungal Genetics and Biology : FG & B·Brisa RamosJosé María Díaz-Mínguez

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