Galactooligosaccharides and Resistant Starch Altered Microbiota and Short-Chain Fatty Acids in an in vitro Fermentation Study Using Gut Contents of Mud Crab (Scylla paramamosain )

Frontiers in Microbiology
Ngoc Tuan TranShengkang Li

Abstract

Dietary carbohydrates are anaerobically fermented by gut microbiota to short-chain fatty acids (SCFAs), conferring gut health benefits. Of all tested prebiotics, galactooligosaccharides (GOS) and resistant starch (RS) stimulated the SCFA production in mud crab (Scylla paramamosain), a crustacean model, to a greater extent than the other carbohydrates tested. Using in vitro anaerobic fermentation cultures, this study further explored the prebiotic potential of GOS and RS in mud crab by assessing their impacts on gut microbiota changes and SCFA production. Both GOS and RS significantly promoted SCFA production. Bacterial diversity in the GOS group was lower than in the RS or control group. GOS promoted the growth of Bacteroidetes, while RS promoted Tenericutes. A strong positive correlation was found between SCFA production and bacterial abundance; most bacteria per se correlated with each other. The findings demonstrated the prebiotic potential of GOS and RS in mud crab.

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Citations

Apr 20, 2021·International Journal of Food Sciences and Nutrition·Jingjing XuXiaobei Zhan
Jul 3, 2021·Journal of Clinical Medicine·Jiezhong Chen, Luis Vitetta

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Cutadapt
PAST
LEfSe
Hutlab
QIIME
Uparse
Heml
PERMANOVA
UniFrac
SPSS

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