Galactosidases in opening, senescing and water-stressed Sandersonia aurantiaca flowers

Functional Plant Biology : FPB
Erin M O'DonoghueDacey A Ryan

Abstract

Three glycosyl hydrolase family 35 β-galactosidase-encoding cDNAs, SaGAL1 (full-length), SaGAL2 and SaGA3L (both partial), have been isolated from Sandersonia aurantiaca (Hook.) SaGAL1 protein was functionally expressed in E. coli and β-galactosidase identity confirmed by activity assay. All three clones are primarily expressed in tepal tissues of senescing sandersonia flowers. In order to identify relationships between tepal texture and galactose metabolism, cut sandersonia flowers were treated with sucrose, periods of dryness or PEG and parameters associated with galactose metabolism and firmness were monitored. Sucrose supplementation, known to increase tepal firmness, delayed expression of SaGAL1 and SaGAL3 in opening (stage 5) flowers, whereas the response to periods of dryness followed by rehydration depended on the maturity of the flower. These treatments also tended to hasten the onset of processes associated with programmed cell death, monitored by PRT5 (a senescence-associated protease) expression. Galactosidase activity and cell wall galactose content were also affected but in an inconsistent manner. PEG supplied to opening flowers for 1 d followed by water, induced a long period of wilt, and intensive PRT5 expressio...Continue Reading

References

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Citations

Dec 17, 2008·Planta·Erin M O'DonoghueDonald A Hunter
Dec 1, 2007·Functional Plant Biology : FPB·Katrien Le RoyWim Van den Ende
Mar 4, 2008·Journal of Experimental Botany·Wouter G van Doorn, Ernst J Woltering
Aug 21, 2021·Functional & Integrative Genomics·Archana SinghIndrakant Kumar Singh

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Datasets Mentioned

BETA
AK103045

Methods Mentioned

BETA
PCR
protein assay
glycosylation
transgenic

Software Mentioned

PSORT
MegAlign Clustal
Genstat
BLASTP
Instron
Multi Gauge

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