Galacturonic acid inhibits the growth of Saccharomyces cerevisiae on galactose, xylose, and arabinose.

Applied and Environmental Microbiology
Eline H HuisjesAntonius J A van Maris

Abstract

The efficient fermentation of mixed substrates is essential for the microbial conversion of second-generation feedstocks, including pectin-rich waste streams such as citrus peel and sugar beet pulp. Galacturonic acid is a major constituent of hydrolysates of these pectin-rich materials. The yeast Saccharomyces cerevisiae, the main producer of bioethanol, cannot use this sugar acid. The impact of galacturonic acid on alcoholic fermentation by S. cerevisiae was investigated with anaerobic batch cultures grown on mixtures of glucose and galactose at various galacturonic acid concentrations and on a mixture of glucose, xylose, and arabinose. In cultures grown at pH 5.0, which is well above the pK(a) value of galacturonic acid (3.51), the addition of 10 g · liter(-1) galacturonic acid did not affect galactose fermentation kinetics and growth. In cultures grown at pH 3.5, the addition of 10 g · liter(-1) galacturonic acid did not significantly affect glucose consumption. However, at this lower pH, galacturonic acid completely inhibited growth on galactose and reduced galactose consumption rates by 87%. Additionally, it was shown that galacturonic acid strongly inhibits the fermentation of xylose and arabinose by the engineered pentos...Continue Reading

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Citations

Jan 30, 2019·Applied Microbiology and Biotechnology·Kevin SchmitzJ Philipp Benz
Jun 1, 2020·Applied Microbiology and Biotechnology·Luís C MartinsIsabel Sá-Correia
Jan 7, 2021·Bioresource Technology·Deokyeol JeongSoo Rin Kim
May 13, 2021·Biotechnology Letters·Pradeep Puligundla, Chulkyoon Mok
May 18, 2021·Frontiers in Plant Science·Nathaniel M WestrickMehdi Kabbage

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