Abstract
This study has investigated whether galangin, a flavonol derived from Alpinia officinarum Hance and used as food additives in southern China, induces apoptosis in hepatocellular carcinoma cells (HCCs) by activation of the caspase-8 and Bid pathway. The apoptosis of HCCs was evaluated by in situ uptake of propidium iodide and Hoechst 33258. Protein expressions were detected by Western blotting. Caspase-8 activity was measured using colorimetric method. To confirm the galangin-induced apoptotic pathway, inhibition of caspase-8 activity by Z-IETD-FMK, knockdown of Bid expression with siRNA, and overexpression of Bcl-2 in cells were carried out, respectively. The results show that galangin has significantly induced apoptosis in HCC lines. The caspase-8 is activated, and the cleavage of Bid results in the increase in tBid. The galangin-induced apoptosis is attenuated by Z-IETD-FMK, Bid siRNA, and Bcl-2 overexpression, respectively. However, Bcl-2 fails to suppress caspase-8 activation and the cleavage of Bid. This study has demonstrated that galangin induces apoptosis in HCCs by activating caspase 8/t-Bid mitochondrial pathway. Although Bcl-2 overexpression attenuates galangin-mediated apoptosis of HCCs, it is not mediated by the in...Continue Reading
References
Sep 1, 1995·The Journal of Experimental Medicine·D T ChaoS J Korsmeyer
Jun 8, 2001·Genes & Development·X Gao, D Pan
Mar 8, 2003·The Journal of Biological Chemistry·Xiaolan YiZheng Dong
Nov 18, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Young Jin MoonMarilyn E Morris
Aug 10, 2006·The Journal of Biological Chemistry·Ajoy K SamrajIngo Schmitz
Oct 26, 2007·Acta Pharmacologica Sinica·Hai-tao ZhangJian-quan Ma
Apr 1, 2008·Cancer Letters·Manlio TolomeoDaniele Simoni
Aug 12, 2008·Drug Discovery Today·Alan L Harvey
Mar 17, 2010·Current Drug Discovery Technologies·Steven WangYing Huang
Jul 16, 2010·World Journal of Gastroenterology : WJG·Hai-Tao ZhangHui-Ming Liu
Aug 11, 2010·The Journal of Cell Biology·Nicolai FrickerInna N Lavrik
Citations
Oct 26, 2012·Cytotechnology·Shailendra Kapoor
Feb 6, 2014·Archives of Pharmacal Research·Dinesh DevadossArulvasu Chinnasamy
Oct 1, 2014·Toxicology·Yajun WangHui Luo
Feb 10, 2016·BioMed Research International·Chen-Yi LiaoWu-Hsien Kuo
Mar 16, 2016·Nutrients·Yue ZhouHua-Bin Li
Sep 14, 2013·European Journal of Pharmacology·Haitao ZhangHui Luo
Oct 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Lijuan SuHui Luo
Jul 28, 2016·Scientific Reports·Xv LiHaitao Zhang
Jul 14, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhao ZhangHai-Yang Liu
Jun 8, 2018·Pharmacology·Dan LiuYanwen Liu
Sep 7, 2018·Food & Function·Ning LiHui Luo
May 13, 2017·Oncology Reports·Sheau-Yun YuanKan-Jen Tsai
Feb 14, 2013·Molecular Medicine Reports·Sungho Ghil
Jul 28, 2016·Drug and Chemical Toxicology·Merve BacanlıNurşen Başaran
Feb 3, 2019·Lasers in Medical Science·Zhao ZhangHai-Tao Zhang
Sep 5, 2020·Drug Design, Development and Therapy·Xinxu ZhangYing Pu
Jan 2, 2019·Cancers·Mariam AbotalebDietrich Büsselberg
Aug 15, 2014·Oncology Letters·Yajun WangBin Wu
Feb 28, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Lucas Amaral-MachadoEryvaldo Sócrates T Egito
Oct 20, 2020·Nutrition and Cancer·Deepa S Mandlik, Satish K Mandlik
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dengyang FangRunlan Luo
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nabil M Abdel-HamidDaniah Muneam Hamid
May 25, 2021·Journal of Food Biochemistry·Jasmine BabyLekshmi R Nath
Aug 17, 2021·Nutrition and Cancer·Deepa S Mandlik, Satish K Mandlik
Mar 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shimaa A AbassMamdouh Mohammad El-Shishtawy