PMID: 7528924Dec 20, 1994Paper

Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy

Proceedings of the National Academy of Sciences of the United States of America
R R JiT Hökfelt

Abstract

Antisense (AS) oligonucleotides (ONs) to galanin (GAL) were applied to the proximal end of a transected sciatic nerve, allowing their cellular uptake and transport into injured axons. GAL expression in dorsal root ganglia and self-mutilation behavior (autotomy) were then studied. AS-ONs with phosphorothioate or allyl modifications significantly suppressed the axotomy-induced increase in GAL levels, as demonstrated by immunohistochemistry and exaggerated autotomy behavior, whereas no significant effect on GAL mRNA levels could be demonstrated with in situ hybridization. Allyl-ONs were more effective than phosphorothioate-ONs. An AS-ON with three base mismatches did not induce any of the above effects. These results support the view that the inhibition of axotomy-induced GAL up-regulation is related to autotomy.

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Citations

Dec 1, 1995·Cellular and Molecular Neurobiology·K KaskT Bartfai
Sep 22, 1995·Regulatory Peptides·J N Crawley
Nov 8, 2001·European Journal of Pharmacology·Z Wiesenfeld-Hallin, X J Xu
Feb 26, 2000·Trends in Pharmacological Sciences·T A BranchekM W Walker
Oct 9, 2002·Trends in Pharmacological Sciences·Hong-Xiang Liu, Tomas Hökfelt
Nov 20, 2001·Current Opinion in Pharmacology·D WynickS B McMahon
Mar 3, 1999·The European Journal of Neuroscience·J X HaoZ Wiesenfeld-Hallin
Feb 24, 2001·Neuroreport·K H BlakemanT Hökfelt
Apr 10, 2004·Neuroreport·James M Kennedy, Douglas W Zochodne
Feb 3, 1999·Annals of the New York Academy of Sciences·U Langel, T Bartfai
Feb 3, 1999·Annals of the New York Academy of Sciences·Z Wiesenfeld-Hallin, X J Xu
May 15, 1996·The Journal of Clinical Investigation·E NoiriM S Goligorsky
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z Q XuT Hökfelt
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·F E HolmesD Wynick
Apr 7, 2005·Behavioral and Cognitive Neuroscience Reviews·John K Robinson
Oct 29, 2014·The Journal of Pharmacology and Experimental Therapeutics·Cameron S MetcalfH Steve White
Dec 23, 1998·Neuroscience and Biobehavioral Reviews·T Kauppila

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