Galanin expression in the mouse major pelvic ganglia during explant culture and following cavernous nerve transection.

Journal of Molecular Neuroscience : MN
Beatrice M GirardRodney L Parsons

Abstract

Autonomic neurons commonly respond to injury/axotomy with an increased expression of neuropeptides including galanin and pituitary adenylyl cyclase-activating polypeptide (PACAP). The increased peptide expression may enhance neuronal survival and axonal regeneration. Using quantitative (Q) PCR and immunocytochemistry, the present study tested whether galanin expression increased in male mouse major pelvic ganglia (MPG) neurons in response to injury. Galanin transcript expression increased significantly in MPG neurons following 72 h in explant culture and 72 h after unilateral transection of the cavernous nerve. Under both conditions, the increase in galanin transcript levels was greater than the increase in PACAP transcript levels. In control MPG, galanin-IR nerve fibers formed pericellular arrangements around MPG neurons although few galanin-IR cells were evident and many of the galanin-IR cells may be small intensely fluorescent (SIF) cells. In 3-day-cultured MPGs, many more galanin-IR cells and nerve fibers were noted. The increased galanin expression was most apparent in neurons that were also immunoreactive for neuronal nitric oxide synthase, rather than tyrosine hydroxylase. Some explant-cultured MPG neurons exhibited imm...Continue Reading

References

May 1, 1994·Neuroscience·R C SchreiberR E Zigmond
Aug 1, 1996·Cell and Tissue Research·G M MaweR L Parsons
Feb 22, 2003·Cell and Tissue Research·Yewlan WanigasekaraJanet R Keast
Jun 10, 2005·The FEBS Journal·Milan Makwana, Gennadij Raivich
Feb 21, 2006·International Review of Cytology·Janet R Keast
Jul 24, 2007·Progress in Neurobiology·X NavarroAntoni Valero-Cabré
Apr 22, 2010·Journal of Molecular Neuroscience : MN·Beatrice M GirardRodney L Parsons
Apr 30, 2010·Journal of Molecular Neuroscience : MN·John D TompkinsRodney L Parsons

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Citations

Mar 23, 2017·The Journal of Comparative Neurology·Christine M BarryRainer V Haberberger

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