Galanin is localized in sympathetic neurons of the dog liver

The American Journal of Physiology
T O MundingerG J Taborsky

Abstract

Stimulation of canine hepatic nerves releases the neuropeptide galanin from the liver; therefore, galanin may be a sympathetic neurotransmitter in the dog liver. To test this hypothesis, we used immunocytochemistry to determine if galanin is localized in hepatic sympathetic nerves and we used hepatic sympathetic denervation to verify such localization. Liver sections from dogs were immunostained for both galanin and the sympathetic enzyme marker tyrosine hydroxylase (TH). Galanin-like immunoreactivity (GALIR) was colocalized with TH in many axons of nerve trunks as well as individual nerve fibers located both in the stroma of hepatic blood vessels and in the liver parenchyma. Neither galanin- nor TH-positive cell bodies were observed. Intraportal 6-hydroxydopamine (6-OHDA) infusion, a treatment that selectively destroys hepatic adrenergic nerve terminals, abolished the GALIR staining in parenchymal neurons but only moderately diminished the GALIR staining in the nerve fibers around blood vessels. To confirm that 6-OHDA pretreatment proportionally depleted galanin and norepinephrine in the liver, we measured both the liver content and the hepatic nerve-stimulated spillover of galanin and norepinephrine from the liver. Pretreatme...Continue Reading

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Apr 14, 1997·Journal of the Autonomic Nervous System·T O MundingerG J Taborsky

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Citations

Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·M ShiotaA D Cherrington
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·T O Mundinger, G J Taborsky

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