Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion.

Molecular Cancer
L Gerard ToussaintJoon H Uhm

Abstract

High-grade gliomas, including glioblastomas (GBMs), are recalcitrant to local therapy in part because of their ability to invade the normal brain parenchyma surrounding these tumors. Animal models capable of recapitulating glioblastoma invasion may help identify mediators of this aggressive phenotype. Patient-derived glioblastoma lines have been propagated in our laboratories and orthotopically xenografted into the brains of immunocompromized mice. Invasive cells at the tumor periphery were isolated using laser capture microdissection. The mRNA expression profile of these cells was compared to expression at the tumor core, using normal mouse brain to control for host contamination. Galectin-1, a target identified by screening the resulting data, was stably over-expressed in the U87MG cell line. Sub-clones were assayed for attachment, proliferation, migration, invasion, and in vivo tumor phenotype. Expression microarray data identified galectin-1 as the most potent marker (p-value 4.0 x 10-8) to identify GBM cells between tumor-brain interface as compared to the tumor core. Over-expression of galectin-1 enhanced migration and invasion in vitro. In vivo, tumors expressing high galectin-1 levels showed enhanced invasion and decrea...Continue Reading

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Nov 26, 2013·Molecular Neurobiology·Eli T SayeghAndrew T Parsa
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Methods Mentioned

BETA
surgical resection
xenograft
xenografts
chip
PCR
transfection
FACS
dissection
xenografting
GTPase

Software Mentioned

Scion Image
GeneSpring
GeneChip

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