Galectin-1 enhances TNFα-induced inflammatory responses in Sertoli cells through activation of MAPK signalling

Scientific Reports
Tao LeiMonika Fijak

Abstract

Galectin-1 (Gal-1) is a pleiotropic lectin involved in the modulation of immune responses. Using a model of rat experimental autoimmune orchitis (EAO), we investigated the role of Gal-1 in testicular inflammation. EAO is characterized by leukocytic infiltrates in the interstitium, damage of spermatogenesis and production of inflammatory mediators like TNFα and MCP1 causing infertility. In normal rat testis Gal-1 was mainly expressed in Sertoli cells and germ cells. In the inflamed testis, Gal-1 expression was significantly downregulated most likely due to germ cell loss. Analyses of lectin binding and expression of glucosaminyl- and sialyltransferases indicated that the glycan composition on the cell surface of Sertoli and peritubular cells becomes less favourable for Gal-1 binding under inflammatory conditions. In primary Sertoli cells Gal-1 expression was found to be upregulated after TNFα challenge. Pretreatment with Gal-1 synergistically and specifically enhanced TNFα-induced expression of MCP1, IL-1α, IL-6 and TNFα in Sertoli cells. Combined stimulation of Sertoli cells with Gal-1 and TNFα enhanced the phosphorylation of MAP kinases as compared to TNFα or Gal-1 alone. Taken together, our data show that Gal-1 modulates infl...Continue Reading

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Citations

Jul 19, 2019·Cell Death & Disease·Fei-Da NiWan-Xi Yang
Apr 23, 2019·Journal of Experimental Neuroscience·William F AbelAnna V Blenda
Dec 31, 2020·International Journal of Molecular Sciences·Jialei GongYong-Gang Duan
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Nov 6, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Ming-Tsai ChiangLee-Young Chau
May 5, 2021·Lung·Miriana d'AlessandroDavid Bennett
May 18, 2021·Asian Journal of Urology·Melanie R MüllerDaniel Nettersheim

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Methods Mentioned

BETA
flow cytometry
Protein Assay
electrophoresis
PCR

Software Mentioned

FlowJo
GraphPad Prism
GraphPad

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