PMID: 9546655Apr 18, 1998Paper

Galectin-1 from ovine placenta--amino-acid sequence, physicochemical properties and implications in T-cell death

European Journal of Biochemistry
M M IglesiasC Wolfenstein-Todel

Abstract

In the present study we report the amino-acid sequence, carbohydrate specificity and overall biochemical and physicochemical properties of galectin-1, a beta-galactoside-binding lectin from ovine placenta. The complete amino-acid sequence, obtained by tryptic and chymotryptic digestion, revealed that this carbohydrate-binding protein shares all the absolutely preserved and critical residues found in other members of the mammalian galectin-1 subfamily. Moreover, conformational changes induced by protein interaction with its specific disaccharide were investigated by fourth-derivative spectral analysis, intrinsic tryptophan fluorescence measurements and circular dichroism. The first two methods indicated changes in the environment of aromatic residues, in agreement with the role of Trp in carbohydrate binding. The quenching of the fluorescence emission upon addition of lactose, allowed us to calculate the Kd for its interaction with the galectin, which was 0.157 +/- 0.02 mM. The far-ultraviolet CD spectra is consistent with the large extent of beta-sheet structure described for other galectins. Addition of lactose produced no significant changes, suggesting that it causes no modifications in the secondary structure of the lectin....Continue Reading

Citations

Dec 31, 2003·Developmental and Comparative Immunology·Satoshi TasumiYuzuru Suzuki
Sep 12, 2002·Biochimica Et Biophysica Acta·Gabriel A RabinovichMarta A Toscano
May 22, 2003·Biochimica Et Biophysica Acta·María Mercedes IglesiasCarlota Wolfenstein-Todel
Aug 3, 1999·The Journal of Experimental Medicine·G A RabinovichY Chernajovsky
May 25, 2005·Stem Cells and Development·T KadriD Lutomski
Jul 4, 2012·Glycobiology·Rosanna E RamhorstGabriel A Rabinovich
Jan 9, 2007·Cancer Letters·Syed Saif HasanNaheed Banu
Feb 15, 2013·American Journal of Reproductive Immunology : AJRI·Ada G Blidner, Gabriel A Rabinovich
Feb 20, 2013·American Journal of Reproductive Immunology : AJRI·Ana Claudia Zenclussen
Apr 2, 2014·Biochimica Et Biophysica Acta·Dolores SolísHans-Joachim Gabius
May 24, 2011·International Immunopharmacology·Roberto C DavicinoGabriel A Rabinovich
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G A RabinovichC E Sotomayor
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G A RabinovichC E Sotomayor
Jun 14, 2006·Glycobiology·Verónica A BironCarlota Wolfenstein-Todel
Jun 24, 2008·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Nandor Gabor ThanRoberto Romero
Oct 14, 2009·Molecular BioSystems·Sabato D'AuriaVanya Bogoeva
Jan 27, 2021·Glycobiology·Anabela M CutineGabriel A Rabinovich
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.