Galectin-1 Modulates the Survival and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) Sensitivity in Human Hepatocellular Carcinoma Cells

Cancer Biotherapy & Radiopharmaceuticals
Juan LiQuancheng Kan

Abstract

Galectin-1 is a member of carbohydrate-binding proteins and plays critical roles in tumor growth and progression. It has been reported that galectin-1 is upregulated in human hepatocellular carcinoma (HCC) and facilitates HCC cell migration and invasion. In this study, the authors aimed to explore the effects of the knockdown of galectin-1 on HCC cell survival and sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Human HCC cells were transfected with galectin-1-targeting small interfering RNA (siRNA) with or without 100 ng/mL TRAIL treatment and tested for apoptosis and gene expression changes. Cotransfection of Bcl-2- and survivin-expressing plasmids with galectin-1 siRNA was done, before TRAIL exposure, cell viability, and apoptosis were assessed. The authors found that siRNA-mediated downregulation of galectin-1 caused apoptosis in HCC cells, which was coupled with reduced Bcl-2 and survivin and increased Bax expression. Overexpression of Bcl-2 and survivin significantly blocked galectin-1 silencing-induced apoptosis of HCC cells. Knockdown of galectin-1 significantly enhanced TRAIL cytotoxicity against HCC cells, as determined by the MTT assay. Moreover, galectin-1 downregulation significantly ...Continue Reading

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Citations

Mar 3, 2018·International Journal of Molecular Sciences·Olivier Micheau
Aug 1, 2020·Journal of Molecular Histology·Mei-Juan SunPing Leng
Mar 9, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jichun SunXiaoxin Jin
Mar 14, 2021·World Journal of Surgical Oncology·Renjie LiuDousheng Bai

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Methods Mentioned

BETA
PCR
fluorescence microscopy
electrophoresis
transfection
flow cytometry

Software Mentioned

Quantity One

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