Galectin-1 Restores Immune Tolerance to Liver Transplantation Through Activation of Hepatic Stellate Cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zhijun JiangShu-sen Zheng

Abstract

Immune tolerance is considered the only way to manage liver transplantation (LT). The current study hypothesized that galectin-1 via the activation of hepatic stellate cells (HSCs) is capable of inducing immune tolerance in LT. Lentiviral-mediated gene knockdown and overexpression of galectin-1 were conducted in HSC-T6 cells. Reverse transcription quantitative polymerase chain reaction and western blot analysis were used to determine galectin-1 expression. LT was performed in 20 C57BL/J6 mice and 20 C3H mice. T-cells were assigned into control, Galectin-1 shRNA, Galectin-1 OE, Galectin-1 OE SB431542, Galectin-1 OE Sulforaphane, Galectin-1 OE Y27632, and Galectin-1 OE UO126 groups. CFSE, flow cytometry, and ELISA were respectively employed to detect T-cell proliferation, CD4+/ CD8+ ratio and IL-2, IL-10 and TGF-β levels. After establishing mouse models of immune tolerance and acute rejection, immunohistochemistry, TUNEL, and immunofluorescence assay were performed to determine CD3+ expression, apoptosis, α-SMA, and desmin. Mouse models of CCl4-induced liver fibrosis were established, followed by assigning the control1 and CCl4 groups. ELISA was used to determine ALT, AST, TBIL and Hyp levels. A total of 3 C57BL/J6 mice (donor) a...Continue Reading

Citations

Aug 1, 2020·Journal of Molecular Histology·Mei-Juan SunPing Leng
Aug 8, 2020·Nature Reviews. Gastroenterology & Hepatology·Angus W ThomsonAlberto Sanchez-Fueyo
Jun 26, 2020·Frontiers in Immunology·Helong DaiNatasha M Rogers
Jan 11, 2020·Frontiers in Oncology·Wei MuMargot Zöller
May 5, 2021·Lung·Miriana d'AlessandroDavid Bennett
May 12, 2021·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Hao LiYing-Zi Ming

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