Galectin-3 Deficiency Facilitates TNF-α-Dependent Hepatocyte Death and Liver Inflammation in MCMV Infection

Frontiers in Microbiology
Bojana StojanovicMarija Milovanovic

Abstract

Galectin-3 (Gal-3) has a role in multiple inflammatory pathways. Various, opposite roles of Gal-3 in liver diseases have been described but there are no data about the role of Gal-3 in development of hepatitis induced with cytomegalovirus infection. In this study we aimed to clarify the role of Gal-3 in murine cytomegalovirus (MCMV)-induced hepatitis by using Gal-3-deficient (Gal-3 KO) mice. Here we provide the evidence that Gal-3 has the protective role in MCMV-induced hepatitis. Enhanced hepatitis manifested by more inflammatory and necrotic foci and serum level of ALT, enhanced apoptosis and necroptosis of hepatocytes and enhanced viral replication were detected in MCMV-infected Gal-3 deficient mice. NK cells does not contribute to more severe liver damage in MCMV-infected Gal-3 KO mice. Enhanced expression of TNF-α in the hepatocytes of Gal-3 KO mice after MCMV infection, abrogated hepatocyte death, and attenuated inflammation in the livers of Gal-3 KO mice after TNF-α blockade suggest that TNF-α plays the role in enhanced disease in Gal-3 deficient animals. Treatment with recombinant Gal-3 reduces inflammation and especially necrosis of hepatocytes in the livers of MCMV-infected Gal-3 KO mice. Our data highlight the protec...Continue Reading

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Citations

Sep 19, 2019·Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry·Humaira B NoorMd A T Sagor
Jul 28, 2020·International Journal of Molecular Sciences·Aleksandar ArsenijevicMarija Milovanovic
Mar 24, 2021·Immunology Letters·Ivan M Srejovic, Miodrag L Lukic
Apr 6, 2021·Frontiers in Pharmacology·Milica VelickovicNebojsa Arsenijevic

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Methods Mentioned

BETA
light microscopy
ELISA
Infection
flow cytometry

Software Mentioned

SPSS
FlowJo

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