Galectin-3 enhances epithelial cell adhesion and wound healing in rat cornea

Ophthalmic Research
C YabutaM Azuma

Abstract

To investigate if galectin-3: (1) enhances adhesion of rat corneal epithelial cells onto a collagen IV substrate and (2) promotes wound healing in rat corneal explants. Primary cultures of rat corneal epithelial cells were fixed and immunostained with galectin-3 antibody. To test cellular adherence onto plates coated with collagen type IV, isolated corneal epithelial cells from rats were cultured for 24 h with or without recombinant galectin-3. The attached cells were counted after fixing and staining with 0.1% crystal violet. Direct binding of galectin-3 to collagen IV was tested using a biotin label transfer method. To evaluate wound healing, explants with a 3.5-mm diameter wound in the central corneal epithelium from rats were incubated for 16 h with or without recombinant galectin-3. Changes in the size of the wound were measured with a digital microscope after staining with 5% fluorescein sodium. In rat corneal epithelial cells, galectin-3 was stained throughout the cytoplasm, with increasing density adjacent to the plasma membrane. Exogenous galectin-3, but not epidermal growth factor (EGF), significantly promoted adhesion of corneal epithelial cells onto the collagen IV substrate. Galectin-3 directly bound to collagen IV...Continue Reading

Citations

Jun 11, 2015·European Journal of Cell Biology·Ellena HönigRalf Jacob
Jun 15, 2015·Experimental Eye Research·Atsuko FujiiMitsuyoshi Azuma
Aug 7, 2014·Tissue Barriers·Mireille ViguierFrédérique Deshayes
Feb 7, 2017·Chembiochem : a European Journal of Chemical Biology·Johann DionCyrille Grandjean
Jan 27, 2018·International Journal of Molecular Sciences·Salvatore SciacchitanoAlberto Ricci
Feb 1, 2018·International Journal of Molecular Sciences·Yi-Chen ChanChun-Hung Lin
Sep 7, 2014·The Journal of Pharmacology and Experimental Therapeutics·Liu-cheng LiJian Gao
Mar 24, 2021·Immunology Letters·Ivan M Srejovic, Miodrag L Lukic
May 18, 2021·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·T Jeethy RamK Sujathan

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