Galectin-3 in urine of cancer patients: stage and tissue specificity

Journal of Cancer Research and Clinical Oncology
Kiruthika BalasubramanianShylaja M Dharmesh

Abstract

Galectin-3 has been implicated in advanced stage of cancer disease. In the current study we examined the possibility of urinary galectin-3 levels to stage cancer disease and to follow up therapy. Urine was collected from all types of cancer patients at different stages including patients undergoing radio/chemotherapy. Galectin-3 level was determined by anti-galectin-3 based ELISA and agglutination assays. Immunoblotting and purification on lactosyl affinity column further confirmed the presence of galectin-3. Cancer samples exhibited stage dependent expression of galectin-3 approx. ranging from 1.0 to 3.3, 4.4 to 5.4, 5.4 to 24.7, 13.1 to 31.9, 13.9 to 32.9 ng/mg C (creatinine) for stage I-V, respectively, at P approximately <0.05 level. Galectin-3 levels were decreased by approx. threefolds after 5th day of therapy. Sample collection being simple and non-invasive, urinary galectin-3 may be used as a potential diagnostic tool for monitoring or follow up of the stage of cancer disease.

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Citations

Feb 18, 2011·International Journal of Clinical Oncology·Min Kyu KimDuk-Soo Bae
May 8, 2013·European Journal of Heart Failure·Rudolf A de BoerBurkert Pieske
May 29, 2015·Journal of Pathology and Translational Medicine·Nandor Gabor ThanChong Jai Kim
Mar 22, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Junxiu LiuShuzhong Yao
May 9, 2014·Chemico-biological Interactions·Sindhuja H Natarajamurthy, Shylaja M Dharmesh
Apr 20, 2010·Cancers·Vitaly BalanA Raz
Sep 23, 2014·Journal of the American Heart Association·Wouter C MeijersRudolf A de Boer
Feb 6, 2017·Clinical & Experimental Metastasis·Sathisha U VenkateshaiahShylaja M Dharmesh

Related Concepts

Mammary Neoplasms, Human
Cervix Carcinoma
Enzyme-Linked Immunosorbent Assay
Malignant Neoplasm of Esophagus
Hemagglutination Test
TNM Staging System
Malignant Neoplasms
Ovarian Neoplasm
Statistical Sensitivity
Galectin 3

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