Galectin-3-independent Down-regulation of GABABR1 due to Treatment with Korean Herbal Extract HAD-B Reduces Proliferation of Human Colon Cancer Cells

Journal of Pharmacopuncture
Kyung-Hee KimHwa-Seong Yoo

Abstract

Many efforts have shown multi-oncologic roles of galectin-3 for cell proliferation, angiogenesis, and apoptosis. However, the mechanisms by which galectin-3 is involved in cell proliferation are not yet fully understood, especially in human colon cancer cells. To cluster genes showing positively or negatively correlated expression with galectin-3, we employed human colon cancer cell lines, SNU-61, SNU-81, SNU-769B, SNU-C4 and SNU-C5 in high-throughput gene expression profiling. Gene and protein expression levels were determined by using real-time quantitative polymerase chain reaction (PCR) and western blot analysis, respectively. The proliferation rate of human colon cancer cells was measured by using a 3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Expression of γ-aminobutyric acid B receptor 1 (GABABR1) showed a positive correlation with galectin-3 at both the transcriptional and the translational levels. Downregulation of galectin-3 decreased not only GABABR1 expression but also the proliferation rate of human colon cancer cells. However, Korean herbal extract, HangAmDan-B (HAD-B), decreased expression of GABABR1 without any expressional change of galectin-3, and offset γ-aminobutyric acid (GAB...Continue Reading

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Citations

Mar 15, 2019·Integrative Cancer Therapies·Hwa Jeong KangIn-Cheol Kang
Sep 1, 2018·The American Journal of Chinese Medicine·Bosung KimKi-Tae Ha
Jul 3, 2021·Cells·Dorota M Radomska-LeśniewskaPaweł Kamiński

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Methods Mentioned

BETA
PCR
immunoprecipitation
transfection

Software Mentioned

Microarray Database
Affymetrix Data Mining Tool ( DMT )
DMT

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