Galectin-3 modulates the polarized surface delivery of β1-integrin in epithelial cells

Journal of Cell Science
Ellena HönigRalf Jacob

Abstract

Epithelial cells require a precise intracellular transport and sorting machinery to establish and maintain their polarized architecture. This machinery includes β-galactoside-binding galectins for targeting of glycoprotein to the apical membrane. Galectin-3 sorts cargo destined for the apical plasma membrane into vesicular carriers. After delivery of cargo to the apical milieu, galectin-3 recycles back into sorting organelles. We analysed the role of galectin-3 in the polarized distribution of β1-integrin in MDCK cells. Integrins are located primarily at the basolateral domain of epithelial cells. We demonstrate that a minor pool of β1-integrin interacts with galectin-3 at the apical plasma membrane. Knockdown of galectin-3 decreases apical delivery of β1-integrin. This loss is restored by supplementation with recombinant galectin-3 and galectin-3 overexpression. Our data suggest that galectin-3 targets newly synthesized β1-integrin to the apical membrane and promotes apical delivery of β1-integrin internalized from the basolateral membrane. In parallel, knockout of galectin-3 results in a reduction in cell proliferation and an impairment in proper cyst development. Our results suggest that galectin-3 modulates the surface dist...Continue Reading

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Citations

Feb 16, 2019·Antioxidants & Redox Signaling·David J R FultonScott A Barman
Oct 18, 2019·Traffic·Sadia RahmaniCostin N Antonescu
Aug 28, 2020·Biomolecules·Sebastian Bänfer, Ralf Jacob
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Apr 18, 2021·The FEBS Journal·Daniel S Levic, Michel Bagnat
Jun 12, 2021·Communications Biology·Laetitia SeguinÉrika Cosset

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