Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling

The Journal of Investigative Dermatology
Hung-Lin ChenFu-Tong Liu

Abstract

Galectin-7, a member of the β-galactoside-binding protein family, is primarily expressed in stratified epithelial cells, including keratinocytes. There is information in the literature suggesting a role for this protein in regulation of keratinocyte survival and growth, but the underlying mechanism remains relatively unknown. Moreover, its expression pattern in the epidermis suggests that it is also involved in the regulation of keratinocyte differentiation. Here, we demonstrate that galectin-7 knockdown results in reduced differentiation and increased proliferation of keratinocytes. Using microarray and deep-sequencing analyses, we found that galectin-7 positively and negatively regulates microRNA (miR)-203 and miR-146a expression, respectively. We show that galectin-7 regulates keratinocyte differentiation and proliferation through miR-203 but not miR-146a. A knockdown of either galectin-7 or miR-203 in keratinocytes increases expression of p63, an essential transcription factor involved in skin development. Rescue of miR-203 expression in a galectin-7 knockdown model reduces p63 expression to baseline. Increased galectin-7 expression upregulates c-Jun N-terminal kinase (JNK) protein levels, which is required for miR-203 expr...Continue Reading

Citations

Sep 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhao XiaohongWang Shaosheng
Mar 27, 2019·Medicinal Research Reviews·Qinghua WuKamil Kuca
Dec 25, 2019·The Journal of Experimental Medicine·María Romina GirottiGabriel A Rabinovich
Dec 2, 2017·Cell Death & Disease·Chun-Xiao LiZhi-Rong Yao
Feb 11, 2018·Experimental Dermatology·Nan-Lin Wu, Fu-Tong Liu
Aug 19, 2020·International Journal of Molecular Sciences·Ai-Young Lee
Aug 26, 2016·BMC Cancer·Juan Carlos Higareda-AlmarazBladimiro Rincon-Orozco
Dec 20, 2017·International Journal of Molecular Sciences·Tamara AdvedissianMireille Viguier
May 12, 2018·Molecular Medicine Reports·Jiaying LiuBaolin Li
Jul 9, 2020·Frontiers in Bioengineering and Biotechnology·Alexis LaurentLee Ann Applegate
Mar 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Apr 18, 2021·Cytokine & Growth Factor Reviews·Natalia PorębskaŁukasz Opaliński
Sep 5, 2020·Burns : Journal of the International Society for Burn Injuries·Weiwei YuanLi Yu
Jun 22, 2021·Histochemistry and Cell Biology·Anna-Kristin LudwigHans-Joachim Gabius
Jul 25, 2021·International Journal of Molecular Sciences·Manabu YoshiokaMotonobu Nakamura
Aug 29, 2021·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chao-Yen HuangTaichen Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
ubiquitination
confocal-microscopy
two-hybrid
immunoprecipitation
transfection
Assay

Software Mentioned

Prism
MetaMorph
Volocity

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis