Gallic acid inhibits the release of ADAMTS4 in nucleus pulposus cells by inhibiting p65 phosphorylation and acetylation of the NF-κB signaling pathway

Oncotarget
Yao HuangJin Fan

Abstract

This study investigated the inhibitory effect of gallic acid (GA) on the release of A Disintegrin and Metalloproteinase with Thrombospondin motifs 4 (ADAMTS4) through the regulation of the NF-κB signaling pathway, which is closely related to the matrix metalloproteinases in nucleus pulposus cells. Different concentrations of GA were added to TNF-α-induced human nucleus pulposus cells (hNPCs) and intervertebral disc degeneration rat model. ADAMTS-4 expression increased both in the TNF-α-induced nucleus pulposus cells and intervertebral disc degeneration rat model. By contrast, the release of ADAMTS-4 was reduced, and the TNF-α-induced apoptosis of nucleus pulposus cells was significantly inhibited after addition of GA at different concentrations. Further study found that the levels of phosphorylated p65 (p-p65) was increased and the classical NF-κB signal pathway was activated after the nucleus pulposus cells were stimulated by TNF-α. Meanwhile, GA suppressed the p65 phosphorylation and inceased p65 deacetylation levels. As a consequence, GA can decrease the expression of ADAMTS-4 in nucleus pulposus cells by regulating the phosphorylation and acetylation of p65 in NF-κB signaling pathways.

References

Jan 26, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·F RannouS Poiraudeau
Apr 20, 2005·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Mechthild StoeckelhuberUlrich Welsch
Nov 6, 2009·Cellular & Molecular Immunology·Soly WangXiaoren Zhang
Nov 15, 2012·Sub-cellular Biochemistry·Muthu K Shanmugam, Gautam Sethi
Feb 2, 2013·The Spine Journal : Official Journal of the North American Spine Society·Nam V VoJames D Kang
Nov 29, 2014·AJNR. American Journal of Neuroradiology·W BrinjikjiJ G Jarvik
Dec 1, 2014·Cell Biochemistry and Biophysics·Zhongyi SunJiwei Tian
Jul 16, 2015·Pharmaceutical Patent Analyst·Sneha ChoubeyVikas Beniwal
Sep 15, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Si TanPeter Nick
Sep 22, 2015·European Cells & Materials·Z I JohnsonM V Risbud
Oct 7, 2015·Critical Reviews in Analytical Chemistry·Felipe Hugo Alencar Fernandes, Hérida Regina Nunes Salgado
Jan 18, 2016·Cytokine & Growth Factor Reviews·Yunes PanahiBehrang Shiri Varnamkhasti
Apr 9, 2016·Cells·Amanda L Rinkenbaugh, Albert S Baldwin
Nov 20, 2016·Acta Biochimica Et Biophysica Sinica·Cheng WangWenjun Wang
Mar 16, 2017·Apoptosis : an International Journal on Programmed Cell Death·Songfeng ChenMin Cui

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Citations

Aug 17, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Hai-Wei ChenXue-Wen Kang

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Methods Mentioned

BETA
ELISA
dissect
acetylation
flow cytometry
FACS
ubiquitination
enzyme-linked immunosorbent assay
electrophoresis
immunoprecipitations

Software Mentioned

Flowjo

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis