Gallium nitrate accelerates partial thickness wound repair and alters keratinocyte integrin expression to favor a motile phenotype

The Journal of Surgical Research
John GoncalvesLisa Staiano-Coico

Abstract

The nitrate form of the Group III transitional element gallium (GN) increases expression of specific structural components of the provisional wound matrix (i.e., collagen type I, fibronectin) in human dermal fibroblasts. To evaluate the potential of GN as a therapeutic option in management of cutaneous trauma, GN-treated partial thickness porcine wounds and experimentally "injured" human keratinocyte (NHK) monolayer cultures were compared with mirror image control (i.e., saline-treated) sites. GN suppressed cell proliferation in both models, as determined by reduced Ki-67 reactivity and significant lengthening of keratinocyte cell cycle transit times, while effectively promoting reepithelialization. The primary effect of GN was apparently to promote cell migration, as neither epidermal thickness nor epidermal differentiation was altered as a result of GN exposure in vivo or in vitro. Significantly enhanced epidermal reepithelialization was associated with alterations in expression of several keratinocyte integrin subunits. GN induced a significant increase in alpha5 expression. alpha5beta1 switching is a characteristic of the motile phenotype in the setting of cutaneous injury. Concomitantly, GN treatment also induced a dramati...Continue Reading

References

Mar 1, 1991·Bone and Mineral·R DonnellyA L Boskey
Mar 1, 1990·Bone and Mineral·T J Hall, T J Chambers
Nov 1, 1988·Calcified Tissue International·M A RepoR P Warrell
Aug 1, 1985·The Journal of Investigative Dermatology·E J O'KeefeD T Woodley
Feb 1, 1995·The Journal of Investigative Dermatology·E C BullenE W Howard
Aug 1, 1994·The Journal of Investigative Dermatology·J GailitR A Clark
Aug 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M P MarinkovichR E Burgeson
Sep 1, 1993·The Journal of Experimental Medicine·L Staiano-CoicoM R Madden
Jan 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P T GuidonR S Bockman
Jul 1, 1993·The Journal of Investigative Dermatology·A B WysockiF Grinnell
Mar 1, 1996·Nature Medicine·J RomerK Dano
Oct 20, 1998·American Journal of Surgery·W K StadelmannG R Tobin
Mar 23, 2000·Biochimie·F S PanagakosP Guidon
Jan 1, 1997·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Dorne R YagerI Kelman Cohen

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Citations

Aug 21, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A BautersM Rogosnitzky
Jun 20, 2020·PloS One·Shauna P LawlessCanaan M Whitfield-Cargile
Jan 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sara PourshahrestaniMark R Towler
May 28, 2019·APL Bioengineering·Stacy CereceresElizabeth Cosgriff-Hernandez

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