Gambicin: a novel immune responsive antimicrobial peptide from the malaria vector Anopheles gambiae

Proceedings of the National Academy of Sciences of the United States of America
Jacopo VizioliGeorge Dimopoulos

Abstract

A novel mosquito antimicrobial peptide, gambicin, and the corresponding gene were isolated in parallel through differential display-PCR, an expressed sequence tag (EST) project, and characterization of an antimicrobial activity in a mosquito cell line by reverse-phase chromatography. The 616-bp gambicin ORF encodes an 81-residue protein that is processed and secreted as a 61-aa mature peptide containing eight cysteines engaged in four disulfide bridges. Gambicin lacks sequence homology with other known proteins. Like other Anopheles gambiae antimicrobial peptide genes, gambicin is induced by natural or experimental infection in the midgut, fatbody, and hemocyte-like cell lines. Within the midgut, gambicin is predominantly expressed in the anterior part. Both local and systemic gambicin expression is induced during early and late stages of natural malaria infection. In vitro experiments showed that the 6.8-kDa mature peptide can kill both Gram-positive and Gram-negative bacteria, has a morphogenic effect on a filamentous fungus, and is marginally lethal to Plasmodium berghei ookinetes. An oxidized form of gambicin isolated from the cell line medium was more active against bacteria than the nonoxidized form from the same medium.

References

Aug 1, 1992·The Journal of Cell Biology·M E SelstedA J Ouellette
Nov 1, 1995·Experimental Parasitology·H M MüllerA Crisanti
Jul 1, 1995·Insect Biochemistry and Molecular Biology·C LowenbergerJ A Hoffmann
Aug 1, 1994·Insect Molecular Biology·G FaviaC Louis
Feb 1, 1996·The American Journal of Tropical Medicine and Hygiene·J DemaioJ C Beier
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·G DimopoulosC Louis
Oct 8, 1997·The EMBO Journal·A M RichmanF C Kafatos
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·M J LehaneS M Lehane
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·G DimopoulosF C Kafatos
Jan 28, 1998·Annual Review of Entomology·J C Beier
Jul 14, 1998·Insect Molecular Biology·C K Moreira-FerroO Marinotti
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Uttenweiler-JosephP Bulet
Jul 30, 1999·Developmental and Comparative Immunology·P BuletD Hoffmann
Apr 27, 2000·Parasitology Today·A GhoshM Jacobs-Lorena
May 4, 2000·Current Topics in Microbiology and Immunology·M MeisterJ A Hoffmann
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·G DimopoulosF C Kafatos
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·A DanielliF C Kafatos
Oct 12, 2000·Cellular and Molecular Life Sciences : CMLS·D DestoumieuxE Bachère

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Citations

Dec 12, 2012·Cell and Tissue Research·Salvador Hernández-MartínezMario H Rodríguez
Jan 22, 2004·Current Opinion in Immunology·Stéphanie Blandin, Elena A Levashina
Oct 11, 2003·Molecular and Biochemical Parasitology·Lyric C BartholomayBruce M Christensen
Nov 14, 2002·Insect Biochemistry and Molecular Biology·Kendall C KrebsQue Lan
Feb 15, 2003·Trends in Parasitology·Anil GhoshMarcelo Jacobs-Lorena
Dec 11, 2002·Trends in Parasitology·Jacopo Vizioli, Michel Salzet
Jan 25, 2003·Cellular Microbiology·George Dimopoulos
Aug 22, 2002·EMBO Reports·Stéphanie BlandinElena A Levashina
Jun 8, 2011·Antimicrobial Agents and Chemotherapy·Fadia ZaknoonAmram Mor
Feb 20, 2007·Antimicrobial Agents and Chemotherapy·I RadzishevskyA Mor
Jun 19, 2002·Antimicrobial Agents and Chemotherapy·Romanico B G ArrighiJ Grant Burgess
Mar 8, 2008·BMC Evolutionary Biology·Aristeidis ParmakelisJeffrey R Powell
Jan 31, 2007·BMC Genomics·Emma WarrGeorge Dimopoulos
Dec 20, 2007·BMC Genomics·Matthew T AliotaBruce M Christensen
Apr 28, 2010·PLoS Neglected Tropical Diseases·Matthew T AliotaBruce M Christensen
Jul 4, 2008·PloS One·Patrícia AbrantesHenrique Silveira
Feb 24, 2009·PloS One·Tovi LehmannJose' M C Ribeiro
Jun 23, 2006·PLoS Pathogens·Yuemei DongGeorge Dimopoulos
Jan 5, 2012·PLoS Pathogens·Yuemei DongGeorge Dimopoulos
Nov 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Nicholas A HeardGeorge Dimopoulos
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sang Woon ShinAlexander S Raikhel
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stephan MeisterLiangbiao Zheng
May 9, 2007·Infection and Immunity·Naotoshi TsujiKozo Fujisaki
Feb 2, 2016·Developmental and Comparative Immunology·Raúl G SaraivaGeorge Dimopoulos
Feb 24, 2016·Frontiers in Microbiology·Ariane F LacerdaMaria F Grossi-de-Sá
May 26, 2010·Journal of Medical Entomology·Eric CalvoJosé M C Ribeiro
Feb 12, 2013·Trends in Biotechnology·Sibao Wang, Marcelo Jacobs-Lorena
Nov 28, 2012·Developmental and Comparative Immunology·April M ClaytonGeorge Dimopoulos
Dec 24, 2015·Developmental and Comparative Immunology·Julián F Hillyer

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