Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Yan YangXinchen Sun

Abstract

Radiotherapy is a widespread treatment in human solid tumors. However, therapy resistance and poor prognosis are still problems. Gambogic acid (GA), extracted from the dried yellow resin of gamboges, has an anticancer effect against various types of cancer cells. To explore the radiosensitivity of GA on esophageal cancer cell line TE13, cell viability was tested by Cell Counting Kit-8 (CCK-8) assay, colony formation assay was used to assess the effects of GA on the radiosensitivity of TE13, and flow cytometry was performed to meter the percentage of apoptosis. The protein levels of microtubule-associated protein 1 light chain 3 (LC3), caspase3, caspase8, casepase9, pAkt, and p-mammalian target of rapamycin (p-mTOR) were tested using Western blot. The distribution of LC3 was detected by immunofluorescence. Additionally, we also examined reactive oxygen species (ROS) expression by laser scanning confocal microscope (LSCM). The cells were transfected with adenovial vector to monitor the autophagy through the expression of green fluorescent protein (GFP-red fluroscent protein (RFP)-LC3. The rates of apoptotic cells in combined-treated TE13 increased significantly compared with the control groups in accordance with the results of We...Continue Reading

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May 8, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan YangXinchen Sun

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Citations

Feb 18, 2016·Chinese Medical Journal·Jia-Cheng TangXiu-Jun Cai
Jul 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dharambir KashyapAnil K Sharma
Nov 5, 2016·Natural Product Research·Bang DongWei Li
Aug 9, 2017·Journal of Cancer Research and Clinical Oncology·Yong XinGuan Jiang
Nov 20, 2019·Medicinal Research Reviews·Tuhina KhanGiuseppe Campiani
Jul 30, 2019·Frontiers in Pharmacology·Weixiao AnShousong Cao
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Mar 10, 2021·Journal of Cancer Research and Clinical Oncology·Hongfang ZhangShenglin Ma

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